Detailed information NTG1016974

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: DQL06_RS00335
• Genome accession: NZ_LS483321
• Coordinates: 54380..55378 (+)
• Length: 332 a.a.
• NCBI ID: WP_002986679.1
• Uniprot ID: A2RC05
• Organism: Streptococcus pyogenes strain NCTC8314
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 49380..60378

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQL06_RS00320 (NCTC8314_00064)	-	50111..51808 (+)	1698	WP_020904816.1	AIPR family protein	-
DQL06_RS00325 (NCTC8314_00065)	purB	51826..53118 (+)	1293	WP_111689440.1	adenylosuccinate lyase	-
DQL06_RS00330 (NCTC8314_00066)	comR	53249..54160 (+)	912	WP_020904818.1	helix-turn-helix domain-containing protein	Regulator
DQL06_RS09715 (NCTC8314_00067)	comS	54251..54349 (+)	99	WP_020833188.1	quorum-sensing system DWW-type pheromone	Regulator
DQL06_RS00335 (NCTC8314_00068)	ruvB	54380..55378 (+)	999	WP_002986679.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
DQL06_RS00340 (NCTC8314_00069)	-	55516..55953 (+)	438	WP_111689441.1	low molecular weight protein-tyrosine-phosphatase	-
DQL06_RS00345 (NCTC8314_00070)	-	55976..56377 (+)	402	WP_002986671.1	MORN repeat-containing protein	-
DQL06_RS00350 (NCTC8314_00071)	-	56374..58149 (+)	1776	WP_053308140.1	acyltransferase family protein	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37565.11 Da
• Isoelectric Point: 5.3005

>NTDB_id=1016974 DQL06_RS00335 WP_002986679.1 54380..55378(+) (ruvB) [Streptococcus pyogenes strain NCTC8314]
MARILDNNVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEAAKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAVEKAGDLVAILNELEPGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPRIANRLLKRVRDYAQIIGDGIIT
AQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGGPVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQK
AYRHLGYPYQNT

Nucleotide

• Length: 999 bp

>NTDB_id=1016974 DQL06_RS00335 WP_002986679.1 54380..55378(+) (ruvB) [Streptococcus pyogenes strain NCTC8314]
ATGGCTAGAATTTTAGACAATAATGTAATGGGAAATGAGGAGTTTTCGGATCGGACTCTTCGCCCTCAATATCTACACGA
ATATATTGGACAAGATAAAGTTAAGGAACAATTCGCTATTTTTATAGAAGCAGCTAAAAGACGTGACGAATCTTTAGACC
ATGTTTTATTATTTGGACCTCCAGGGCTGGGAAAAACAACAATGGCATTTGTGATTGCCAATGAATTGGGAGTTAATTTA
AAGCAAACTTCAGGTCCTGCCGTTGAGAAAGCTGGAGATTTGGTAGCTATTTTAAATGAATTAGAACCAGGGGATATTTT
GTTTATTGATGAAATTCACCGCATGCCAATGTCTGTCGAGGAAGTACTATATAGTGCTATGGAAGACTTCTATATTGATA
TTATGATTGGCGCTGGAGATACTAGTCGTAGTATTCATTTGGATCTGCCTCCATTTACATTGATTGGTGCTACTACACGT
GCAGGCATGTTATCTAATCCCCTCAGAGCTCGTTTTGGTATCACTGGTCACATGGAATACTATCAAGAAAAGGATTTGAC
AGAGATTGTGGAGCGTACAGCTACTATTTTTGAAATAAAAATAGACCATGAAGCTGCACGGAAATTAGCTTGTCGCAGTA
GAGGAACGCCCCGCATTGCTAATCGATTACTAAAACGAGTGCGTGACTATGCTCAAATTATTGGTGATGGCATCATAACG
GCTCAAATAACTGATAGAGCTTTGACTATGTTAGATGTTGACCGCGAAGGTCTCGATTACATTGATCAGAAAATTTTACG
AACGATGATTGAGATGTATCAGGGAGGGCCAGTTGGTTTGGGGACATTATCTGTGAATATTGCCGAGGAGCGTAATACAG
TTGAAGAGATGTATGAACCCTACTTAATTCAAAAAGGCTTTCTCATGCGAACACGAACAGGTCGTGTAGCGACGCAAAAG
GCTTATCGTCATCTTGGCTATCCCTATCAAAACACTTAA

Domains

Predicted by InterProScan.

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: A2RC05

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae D39	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae TIGR4	84.545	99.398	0.84
ruvB	Bacillus subtilis subsp. subtilis str. 168	58.824	97.289	0.572
ruvB	Helicobacter pylori 26695	48.936	99.096	0.485
ruvB	Synechocystis sp. PCC 6803	50.16	94.277	0.473