Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ABU614_RS02010 Genome accession   NZ_CP159925
Coordinates   462427..463485 (-) Length   352 a.a.
NCBI ID   WP_064746591.1    Uniprot ID   A0AAU8MWK4
Organism   Lysobacter firmicutimachus strain SR10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 457427..468485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABU614_RS01990 (ABU614_01990) pilQ 458273..460297 (-) 2025 WP_363798690.1 type IV pilus secretin PilQ -
  ABU614_RS01995 (ABU614_01995) - 460358..460906 (-) 549 WP_363798692.1 pilus assembly protein PilP -
  ABU614_RS02000 (ABU614_02000) pilO 460908..461627 (-) 720 WP_363798695.1 type 4a pilus biogenesis protein PilO -
  ABU614_RS02005 (ABU614_02005) - 461624..462427 (-) 804 WP_336132522.1 PilN domain-containing protein -
  ABU614_RS02010 (ABU614_02010) pilM 462427..463485 (-) 1059 WP_064746591.1 pilus assembly protein PilM Machinery gene
  ABU614_RS02015 (ABU614_02015) - 463886..466450 (+) 2565 WP_064746592.1 penicillin-binding protein 1A -
  ABU614_RS02020 (ABU614_02020) - 466698..467351 (+) 654 WP_363798698.1 hypothetical protein -
  ABU614_RS02025 (ABU614_02025) - 467500..467892 (+) 393 WP_363798700.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37787.23 Da        Isoelectric Point: 4.6331

>NTDB_id=1016814 ABU614_RS02010 WP_064746591.1 462427..463485(-) (pilM) [Lysobacter firmicutimachus strain SR10]
MGLITKSQPALVGVDISSTAVKLLQLSRVGNRYRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIKRAVARSGTRIKHAAAA
VAGSSVITRVIGMPADLDDDDMESQIELEAANYVPYPIDEVNHDFEILGPMPGSPDMVQVLLAASRSENVEVRASALELG
GLIPKVMDVEAFAIENAFSLLADTLSGPRDGLVALVDSGATMTTLNVLRNGRSLYHREQVFGGKQLTDEVMRRYGLSYEE
AGLAKRQGGLPESYQAEVLEPFKEAMVQQVSRLLQFFYAGSEFNRVDQIVLAGGGASIPRIAEMVEEQLGVPTTVANPLA
HMTLGPRVQAHALAQDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=1016814 ABU614_RS02010 WP_064746591.1 462427..463485(-) (pilM) [Lysobacter firmicutimachus strain SR10]
GTGGGACTCATCACAAAAAGCCAACCGGCGCTGGTCGGTGTGGACATCAGTTCGACCGCGGTAAAGCTGTTGCAACTCTC
TCGAGTGGGCAACCGCTACCGCGTTGAGCATTACGCCGTCGAACCGCTGCCGCCGAACGCGGTAGTGGAGAAGAACATCG
TCGAGGTCGAGGCGGTCGGCGAAGCGATCAAGCGTGCCGTTGCGCGGTCGGGGACCCGCATCAAGCACGCCGCCGCGGCG
GTCGCCGGGTCGTCGGTGATCACCCGCGTGATCGGCATGCCCGCGGATCTCGACGACGACGACATGGAATCGCAGATCGA
GCTGGAGGCGGCCAACTACGTGCCGTATCCGATCGACGAAGTGAACCACGACTTCGAAATCCTCGGCCCGATGCCGGGCA
GCCCGGACATGGTCCAGGTGCTGTTGGCCGCCTCGCGTTCGGAGAACGTCGAAGTGCGCGCCTCGGCGCTCGAGCTCGGC
GGCCTGATCCCCAAGGTGATGGACGTGGAGGCCTTCGCGATCGAGAACGCGTTCTCGTTGCTGGCCGACACGCTCAGCGG
TCCGCGCGACGGTCTGGTCGCCCTGGTCGACTCGGGCGCCACCATGACCACCCTGAACGTCCTGCGCAACGGCCGCAGCC
TCTATCACCGCGAGCAGGTGTTCGGCGGCAAGCAGTTGACCGACGAGGTCATGCGCCGCTATGGCCTGAGCTACGAGGAA
GCCGGTCTGGCCAAGCGCCAGGGCGGTCTGCCCGAGAGCTACCAGGCCGAGGTGCTTGAGCCGTTCAAGGAGGCGATGGT
CCAGCAGGTCAGCCGTCTGTTGCAGTTCTTCTACGCCGGCAGCGAATTCAACCGCGTCGACCAGATCGTCTTGGCCGGCG
GCGGCGCTTCGATCCCGCGCATCGCCGAAATGGTCGAAGAGCAACTGGGCGTGCCGACCACGGTGGCCAACCCGCTCGCG
CACATGACGTTGGGCCCGCGCGTCCAGGCGCATGCCCTGGCGCAGGACGCCCCCGCGCTGATGATCGCTTGCGGCCTGGC
CCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.42

100

0.514
  comM Acinetobacter nosocomialis M2

51.136

100

0.511
  comM Acinetobacter baylyi ADP1

50.284

100

0.503
  pilM Legionella pneumophila strain ERS1305867

44.193

100

0.443

Multiple sequence alignment