Detailed information NTG1016167

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABRY27_RS10070
• Genome accession: NZ_CP159841
• Coordinates: 2058007..2059155 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PA3117
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2053007..2064155

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABRY27_RS10050 (ABRY27_10050)	-	2053744..2054928 (-)	1185	WP_003100953.1	cystathionine gamma-synthase	-
ABRY27_RS10055 (ABRY27_10055)	-	2054925..2056298 (-)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
ABRY27_RS10060 (ABRY27_10060)	-	2056541..2056945 (-)	405	WP_003084560.1	NINE protein	-
ABRY27_RS10065 (ABRY27_10065)	czcD	2057100..2057999 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ABRY27_RS10070 (ABRY27_10070)	pilU	2058007..2059155 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
ABRY27_RS10075 (ABRY27_10075)	pilT	2059333..2060367 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ABRY27_RS10080 (ABRY27_10080)	-	2060582..2061274 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ABRY27_RS10085 (ABRY27_10085)	proC	2061286..2062107 (+)	822	WP_031757004.1	pyrroline-5-carboxylate reductase	-
ABRY27_RS10090 (ABRY27_10090)	-	2062118..2062711 (+)	594	WP_003084544.1	YggT family protein	-
ABRY27_RS10095 (ABRY27_10095)	-	2062708..2063004 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1016167 ABRY27_RS10070 WP_003100959.1 2058007..2059155(-) (pilU) [Pseudomonas aeruginosa strain PA3117]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1016167 ABRY27_RS10070 WP_003100959.1 2058007..2059155(-) (pilU) [Pseudomonas aeruginosa strain PA3117]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382