Detailed information NTG1014191

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABWX12_RS14350
• Genome accession: NZ_CP159521
• Coordinates: 3002550..3003668 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain NAB01B-R5
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2997550..3008668

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABWX12_RS14340 (ABWX12_14340)	-	3000666..3001358 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
ABWX12_RS14345 (ABWX12_14345)	pilT	3001485..3002522 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
ABWX12_RS14350 (ABWX12_14350)	pilU	3002550..3003668 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ABWX12_RS14355 (ABWX12_14355)	fur	3003738..3004175 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
ABWX12_RS14360 (ABWX12_14360)	bamE	3004287..3004685 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
ABWX12_RS14365 (ABWX12_14365)	-	3004740..3005063 (-)	324	WP_000442587.1	RnfH family protein	-
ABWX12_RS14370 (ABWX12_14370)	-	3005063..3006130 (-)	1068	WP_002019572.1	hypothetical protein	-
ABWX12_RS14375 (ABWX12_14375)	-	3006283..3006738 (-)	456	WP_000782976.1	bacteriohemerythrin	-
ABWX12_RS14380 (ABWX12_14380)	-	3006991..3007839 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=1014191 ABWX12_RS14350 WP_000347039.1 3002550..3003668(+) (pilU) [Acinetobacter baumannii strain NAB01B-R5]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=1014191 ABWX12_RS14350 WP_000347039.1 3002550..3003668(+) (pilU) [Acinetobacter baumannii strain NAB01B-R5]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395