Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   JMW03_RS01990 Genome accession   NZ_LR890349
Coordinates   443401..444639 (+) Length   412 a.a.
NCBI ID   WP_000815987.1    Uniprot ID   Q3YWN2
Organism   Escherichia coli strain MSB1_9D isolate MSB1_9D     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 438401..449639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JMW03_RS01965 mrcA 438681..441233 (-) 2553 WP_001352334.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  JMW03_RS01970 hofM 441353..442132 (+) 780 WP_001315880.1 DNA utilization protein HofM -
  JMW03_RS01975 hofN 442132..442671 (+) 540 WP_201514286.1 DNA utilization protein HofN -
  JMW03_RS01980 hofO 442655..443095 (+) 441 WP_001055760.1 DNA utilization protein HofO -
  JMW03_RS01985 hofP 443085..443489 (+) 405 WP_001264141.1 DNA utilization protein HofP -
  JMW03_RS01990 comE 443401..444639 (+) 1239 WP_000815987.1 DNA uptake porin HofQ Machinery gene
  JMW03_RS01995 aroK 445040..445561 (+) 522 WP_000818618.1 shikimate kinase AroK -
  JMW03_RS02000 aroB 445618..446706 (+) 1089 WP_000439850.1 3-dehydroquinate synthase -
  JMW03_RS02005 damX 446798..448084 (+) 1287 WP_000343195.1 cell division protein DamX -
  JMW03_RS02010 dam 448191..449027 (+) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44716.24 Da        Isoelectric Point: 6.3183

>NTDB_id=1014088 JMW03_RS01990 WP_000815987.1 443401..444639(+) (comE) [Escherichia coli strain MSB1_9D isolate MSB1_9D]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNNNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=1014088 JMW03_RS01990 WP_000815987.1 443401..444639(+) (comE) [Escherichia coli strain MSB1_9D isolate MSB1_9D]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGGCAGGAAGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATAACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGCTTGCTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YWN2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  comE Haemophilus influenzae 86-028NP

36.927

100

0.391
  pilQ Vibrio campbellii strain DS40M4

38.005

100

0.388
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.53

100

0.376
  pilQ Vibrio cholerae strain A1552

37.53

100

0.376
  comE Glaesserella parasuis strain SC1401

35.952

100

0.367