Detailed information NTG1014050

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABWW74_RS14365
• Genome accession: NZ_CP159517
• Coordinates: 3002549..3003667 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain NAB04B
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2997549..3008667

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABWW74_RS14355 (ABWW74_14355)	-	3000665..3001357 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
ABWW74_RS14360 (ABWW74_14360)	pilT	3001484..3002521 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
ABWW74_RS14365 (ABWW74_14365)	pilU	3002549..3003667 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ABWW74_RS14370 (ABWW74_14370)	fur	3003737..3004174 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
ABWW74_RS14375 (ABWW74_14375)	bamE	3004286..3004684 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
ABWW74_RS14380 (ABWW74_14380)	-	3004739..3005062 (-)	324	WP_000442587.1	RnfH family protein	-
ABWW74_RS14385 (ABWW74_14385)	-	3005062..3006129 (-)	1068	WP_002019572.1	hypothetical protein	-
ABWW74_RS14390 (ABWW74_14390)	-	3006282..3006737 (-)	456	WP_000782976.1	bacteriohemerythrin	-
ABWW74_RS14395 (ABWW74_14395)	-	3006990..3007838 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=1014050 ABWW74_RS14365 WP_000347039.1 3002549..3003667(+) (pilU) [Acinetobacter baumannii strain NAB04B]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=1014050 ABWW74_RS14365 WP_000347039.1 3002549..3003667(+) (pilU) [Acinetobacter baumannii strain NAB04B]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395