Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABNK30_RS15720 Genome accession   NZ_CP159509
Coordinates   3411260..3412402 (-) Length   380 a.a.
NCBI ID   WP_005408369.1    Uniprot ID   A0A854DW57
Organism   Stenotrophomonas maltophilia strain SM911     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3406260..3417402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNK30_RS15695 - 3407372..3407917 (+) 546 WP_033834834.1 DNA-3-methyladenine glycosylase I -
  ABNK30_RS15700 - 3408001..3408708 (-) 708 WP_014036228.1 YitT family protein -
  ABNK30_RS15705 - 3408819..3409028 (-) 210 WP_111188470.1 DUF4287 domain-containing protein -
  ABNK30_RS15710 - 3409122..3410021 (+) 900 WP_049454184.1 LysR family transcriptional regulator -
  ABNK30_RS15715 - 3410069..3411052 (-) 984 WP_111108625.1 aldo/keto reductase -
  ABNK30_RS15720 pilU 3411260..3412402 (-) 1143 WP_005408369.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABNK30_RS15725 pilT 3412503..3413540 (-) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  ABNK30_RS15730 - 3413628..3414305 (+) 678 WP_111108624.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABNK30_RS15735 proC 3414324..3415145 (+) 822 WP_033834830.1 pyrroline-5-carboxylate reductase -
  ABNK30_RS15740 - 3415205..3416080 (-) 876 WP_353949656.1 LysR family transcriptional regulator -
  ABNK30_RS15745 - 3416170..3416781 (+) 612 Protein_3091 short chain dehydrogenase -
  ABNK30_RS15750 soxR 3416854..3417288 (-) 435 WP_033834828.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42355.69 Da        Isoelectric Point: 6.7683

>NTDB_id=1013933 ABNK30_RS15720 WP_005408369.1 3411260..3412402(-) (pilU) [Stenotrophomonas maltophilia strain SM911]
MNTTATTIDFTSFLKLMAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAI
GLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTG
HIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNA
NQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLG
MKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=1013933 ABNK30_RS15720 WP_005408369.1 3411260..3412402(-) (pilU) [Stenotrophomonas maltophilia strain SM911]
GTGAACACCACCGCGACCACCATCGATTTCACTTCGTTCCTCAAGCTGATGGCGCACCAGCGCGCCTCGGACCTGTTCAT
CACTGCGGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCCGATCACGCAGACCCCGCTCACGCCGCAGCAGA
GCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACCCACGAATGCAACTTCGCCATC
GGCCTGTCCGGTGTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGTCGCATCGA
GACGCGCATTCCGACGGTGGAAGAACTGAGCCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCC
TGTTCGTCGGTGCCACCGGTACCGGTAAATCGACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAACTCGACCGGC
CACATCATCACCATCGAAGATCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCAT
CGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATTGGCGAGGTGCGTA
CCCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAAACCGGCCACCTGGTGCTGTGCACCCTGCATGCCAACAACGCC
AACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGCAACCAGCTGCTGATGGACCTGTCGCTGAATCT
CAAGGGCGTGGTCGCGCAGCAGCTGGTGCCGTCGCCCGATGGCCGCTCGCGCAAGGTGGCGATGGAGATCCTGCTGGGCA
CGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATGAAGGACTCGGTCCAGCTGGGC
ATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGTTACGCCGA
TTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGACGCACGCACGCTGTCACAGGGCCTGGATGGCG
TGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A854DW57

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.847

96.579

0.655
  pilU Acinetobacter baylyi ADP1

63.585

93.947

0.597
  pilU Vibrio cholerae strain A1552

53.371

93.684

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.099

93.421

0.403

Multiple sequence alignment