Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   ABVR74_RS06980 Genome accession   NZ_CP159484
Coordinates   1403458..1404780 (+) Length   440 a.a.
NCBI ID   WP_023189341.1    Uniprot ID   A0A0V8BZU5
Organism   Lactococcus lactis subsp. lactis strain CBA3648     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1398458..1409780
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABVR74_RS06955 (ABVR74_06955) - 1399274..1399921 (-) 648 WP_058223056.1 GIY-YIG nuclease family protein -
  ABVR74_RS06960 (ABVR74_06960) - 1400213..1400959 (-) 747 WP_058223057.1 bifunctional metallophosphatase/5'-nucleotidase -
  ABVR74_RS06965 (ABVR74_06965) - 1401074..1402093 (-) 1020 WP_012897709.1 DUF475 domain-containing protein -
  ABVR74_RS06970 (ABVR74_06970) - 1402141..1402638 (-) 498 WP_023189340.1 VanZ family protein -
  ABVR74_RS06975 (ABVR74_06975) - 1402772..1403401 (-) 630 WP_003130660.1 YigZ family protein -
  ABVR74_RS06980 (ABVR74_06980) comFA 1403458..1404780 (+) 1323 WP_023189341.1 DEAD/DEAH box helicase Machinery gene
  ABVR74_RS06985 (ABVR74_06985) comFC 1405023..1405427 (+) 405 WP_257141021.1 ComF family protein Machinery gene
  ABVR74_RS06990 (ABVR74_06990) - 1405460..1406038 (-) 579 WP_023189343.1 nucleotidyltransferase family protein -
  ABVR74_RS06995 (ABVR74_06995) - 1406052..1406507 (-) 456 WP_003130653.1 diacylglycerol kinase -
  ABVR74_RS07000 (ABVR74_07000) ybeY 1406491..1406979 (-) 489 WP_003130652.1 rRNA maturation RNase YbeY -
  ABVR74_RS07005 (ABVR74_07005) - 1407131..1407610 (-) 480 WP_003130650.1 NUDIX hydrolase -
  ABVR74_RS07010 (ABVR74_07010) - 1407668..1408621 (-) 954 WP_058212272.1 PhoH family protein -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50249.76 Da        Isoelectric Point: 10.1065

>NTDB_id=1013830 ABVR74_RS06980 WP_023189341.1 1403458..1404780(+) (comFA) [Lactococcus lactis subsp. lactis strain CBA3648]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLEAKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDNYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKITSVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=1013830 ABVR74_RS06980 WP_023189341.1 1403458..1404780(+) (comFA) [Lactococcus lactis subsp. lactis strain CBA3648]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAGCGAAGGTAAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCGGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAACTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTACTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(300-403)

Type III restriction enzyme, res subunit (ResIII)

(114-233)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0V8BZU5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74.25

90.909

0.675
  comFA/cflA Streptococcus mitis SK321

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae Rx1

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae D39

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae R6

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae TIGR4

52.01

90.455

0.47
  comFA/cflA Streptococcus mitis NCTC 12261

51.256

90.455

0.464
  comFA Bacillus subtilis subsp. subtilis str. 168

37.029

100

0.38

Multiple sequence alignment