Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   N011_RS11850 Genome accession   NZ_CP159362
Coordinates   2610346..2611410 (+) Length   354 a.a.
NCBI ID   WP_024689062.1    Uniprot ID   -
Organism   Pseudomonas syringae CC1417     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2605346..2616410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N011_RS11840 (N011_11840) - 2606256..2606711 (-) 456 WP_024685741.1 YkgJ family cysteine cluster protein -
  N011_RS11845 (N011_11845) - 2607695..2610139 (-) 2445 WP_024689061.1 penicillin-binding protein 1A -
  N011_RS11850 (N011_11850) pilM 2610346..2611410 (+) 1065 WP_024689062.1 pilus assembly protein PilM Machinery gene
  N011_RS11855 (N011_11855) - 2611410..2611982 (+) 573 WP_024689063.1 PilN domain-containing protein -
  N011_RS11860 (N011_11860) pilO 2611979..2612602 (+) 624 WP_024696158.1 type 4a pilus biogenesis protein PilO -
  N011_RS11865 (N011_11865) - 2612599..2613126 (+) 528 WP_024689065.1 pilus assembly protein PilP -
  N011_RS11870 (N011_11870) pilQ 2613174..2615273 (+) 2100 WP_024689066.1 type IV pilus secretin PilQ Machinery gene
  N011_RS11875 (N011_11875) aroK 2615278..2615796 (+) 519 WP_004882714.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38160.57 Da        Isoelectric Point: 4.7049

>NTDB_id=1013422 N011_RS11850 WP_024689062.1 2610346..2611410(+) (pilM) [Pseudomonas syringae CC1417]
MFELFSKKANTLLGIDISSTSVKLLELSRSGNRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVIVKAKTSARIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLEPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRLGTPTQVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1013422 N011_RS11850 WP_024689062.1 2610346..2611410(+) (pilM) [Pseudomonas syringae CC1417]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTTGAATT
GAGTCGCTCCGGAAACCGCTACAAAGTCGAGTCTTACGCCGTCGAGCCGCTGCCTGCCAATGCCGTGGTCGAGAAGAACA
TTGCCGAGCTCGAAGGGGTCGGCCAGGCGCTGTCACGGGTTATCGTCAAGGCCAAGACCAGCGCCAGAATCGTGGCGGTG
GCCGTGGCAGGTTCCGCGGTCATCACCAAGACCATCGAGATGGATGCCGGCCTGTCGGACGACGACATGGAGAACCAGCT
CAAGCTCGAAGCCGACCAATACATTCCCTATCCGCTGGAAGAAGTCGCCATCGATTTCGAGGTGCAGGGCTATTCGGTGC
GCAACCCCGAGCGTGTCGAGGTGCTGCTGGCGGCCTGTCGCAAGGAGAACGTCGAAGTTCGCGAGGCCGCGCTGGCATTG
GCCGGGCTGACCGCGCGCGTCGTCGACGTGGAGGCTTATGCGCTGGAACGTTCGTTCGGCCTGTTGGCGACGCAATTGGG
CAATGGCCATGACGAGCTGACCGTCGCTGTCGTCGACATCGGCGCGACCATGACCACCCTGAGCGTACTGCACCACGGCC
GCATCATTTATACCCGCGAGCAACTGTTTGGCGGGCGACAGCTGACGGATGAGATCCAGCGGCGCTATGGCCTCTCGATG
GAAGAAGCCGGCCTTGCGAAAAAGCAGGGCGGCCTGCCTGACGACTACGTCAGCGAGGTGCTGGAACCTTTCAAGGATGC
GTTGGTGCAACAGGTCTCGCGTTCCCTGCAGTTCTTCTTCGCGGCAGGTCAGTACAACTCTGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTTGAACATTTGATTCAGCGTCGTCTTGGCACGCCGACCCAGGTAGCCAACCCG
TTTGCTGACATGGCACTGAGTTCCAAGGTCAACGCCGGTGCGCTGGCCAGCGATGCACCAGCCTTGATGATCGCGTGCGG
TCTGGCTTTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

47.042

100

0.472

Multiple sequence alignment