Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   N027_RS02850 Genome accession   NZ_CP159278
Coordinates   588815..589384 (+) Length   189 a.a.
NCBI ID   WP_003426596.1    Uniprot ID   A0A0K8M6E3
Organism   Pseudomonas syringae USA007     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 590334..600138 588815..589384 flank 950


Gene organization within MGE regions


Location: 588815..600138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N027_RS02850 (N027_02850) ssb 588815..589384 (+) 570 WP_003426596.1 single-stranded DNA-binding protein Machinery gene
  N027_RS02855 (N027_02855) - 589482..590309 (-) 828 WP_024658746.1 class I SAM-dependent methyltransferase -
  N027_RS02860 (N027_02860) - 590334..591230 (-) 897 WP_024658747.1 crotonase/enoyl-CoA hydratase family protein -
  N027_RS02865 (N027_02865) - 591559..591858 (-) 300 WP_024658748.1 hypothetical protein -
  N027_RS02870 (N027_02870) - 591981..592724 (-) 744 WP_005614319.1 inositol monophosphatase family protein -
  N027_RS02875 (N027_02875) - 592721..593482 (-) 762 WP_024658749.1 glycerophosphoryl diester phosphodiesterase -
  N027_RS02880 (N027_02880) - 593489..594595 (-) 1107 WP_024658750.1 ABC transporter ATP-binding protein -
  N027_RS02885 (N027_02885) - 594592..595485 (-) 894 WP_024658751.1 ABC transporter permease subunit -
  N027_RS02890 (N027_02890) - 595482..596375 (-) 894 WP_024658752.1 carbohydrate ABC transporter permease -
  N027_RS02895 (N027_02895) - 596372..597823 (-) 1452 WP_024658753.1 extracellular solute-binding protein -
  N027_RS02900 (N027_02900) - 597989..599119 (+) 1131 WP_024658754.1 ROK family transcriptional regulator -
  N027_RS02905 (N027_02905) - 599147..599680 (-) 534 Protein_569 hypothetical protein -
  N027_RS02910 (N027_02910) - 599896..600138 (+) 243 WP_024658756.1 hypothetical protein -

Sequence


Protein


Download         Length: 189 a.a.        Molecular weight: 20974.10 Da        Isoelectric Point: 5.9404

>NTDB_id=1013002 N027_RS02850 WP_003426596.1 588815..589384(+) (ssb) [Pseudomonas syringae USA007]
MARGVNKVILVGTCGQDPEVRYLPNGNAVTNLSLATSEQWTDKQSGQKVEKTEWHRVSMFGKVAEIAGEYLRKGSQVYIE
GKLQTREWEKDGIKRYTTEIVVDMQGTMQLLGGRPQGDAQQGQGGGGNYNQSAPRPQQSRPQQSAPQQSAPQQNYNQQPP
QQRDSRPAPQQQAPQPAADFDSFDDDIPF

Nucleotide


Download         Length: 570 bp        

>NTDB_id=1013002 N027_RS02850 WP_003426596.1 588815..589384(+) (ssb) [Pseudomonas syringae USA007]
ATGGCCCGTGGGGTTAACAAAGTCATATTGGTCGGTACATGCGGCCAGGATCCCGAAGTTCGCTACTTGCCTAACGGTAA
CGCCGTGACCAACCTGAGTCTGGCAACCAGCGAACAATGGACTGACAAGCAGAGCGGCCAGAAGGTCGAGAAGACCGAGT
GGCACCGTGTTTCGATGTTCGGCAAGGTTGCCGAAATCGCCGGCGAATACCTGCGCAAGGGTTCGCAGGTCTACATCGAA
GGCAAGCTGCAGACCCGCGAATGGGAAAAAGACGGCATCAAGCGTTACACCACTGAAATCGTCGTCGACATGCAAGGCAC
CATGCAACTGCTGGGCGGCCGTCCACAGGGCGACGCTCAACAAGGTCAGGGCGGTGGCGGCAACTACAACCAGTCCGCGC
CTCGTCCACAGCAGTCGCGCCCACAGCAATCGGCGCCACAGCAGTCCGCACCTCAGCAGAACTACAACCAGCAGCCGCCA
CAACAGCGCGATTCTCGCCCAGCGCCGCAACAACAAGCGCCGCAGCCTGCTGCTGACTTTGACAGCTTTGATGACGATAT
TCCGTTCTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0K8M6E3

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Vibrio cholerae strain A1552

55.208

100

0.561

  ssb Glaesserella parasuis strain SC1401

47.368

100

0.476

  ssb Neisseria meningitidis MC58

44.681

99.471

0.444

  ssb Neisseria gonorrhoeae MS11

44.681

99.471

0.444


Multiple sequence alignment