Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ABNQ20_RS26175 Genome accession   NZ_CP158051
Coordinates   5646566..5647438 (+) Length   290 a.a.
NCBI ID   WP_003141356.1    Uniprot ID   A0A0H2ZGZ1
Organism   Pseudomonas aeruginosa strain P1110     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5642109..5653166 5646566..5647438 within 0


Gene organization within MGE regions


Location: 5642109..5653166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNQ20_RS26155 (ABNQ20_26150) - 5642109..5642666 (-) 558 WP_225011032.1 hypothetical protein -
  ABNQ20_RS26160 (ABNQ20_26155) - 5642871..5643410 (-) 540 WP_003141352.1 pilin -
  ABNQ20_RS26165 (ABNQ20_26160) pilB 5643639..5645342 (+) 1704 WP_003141354.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABNQ20_RS26170 (ABNQ20_26165) pilC 5645346..5646563 (+) 1218 WP_016254080.1 type II secretion system F family protein Machinery gene
  ABNQ20_RS26175 (ABNQ20_26170) pilD 5646566..5647438 (+) 873 WP_003141356.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ABNQ20_RS26180 (ABNQ20_26175) coaE 5647435..5648046 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  ABNQ20_RS26185 (ABNQ20_26180) yacG 5648043..5648243 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  ABNQ20_RS26190 (ABNQ20_26185) - 5648280..5648489 (-) 210 WP_003094660.1 hypothetical protein -
  ABNQ20_RS26195 (ABNQ20_26190) - 5648595..5649284 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  ABNQ20_RS26200 (ABNQ20_26195) - 5649281..5649751 (-) 471 WP_003094664.1 hypothetical protein -
  ABNQ20_RS26205 (ABNQ20_26200) - 5649836..5651113 (-) 1278 WP_000610830.1 ISL3 family transposase -
  ABNQ20_RS26210 (ABNQ20_26205) - 5651126..5652130 (-) 1005 WP_040363373.1 permease -
  ABNQ20_RS26215 (ABNQ20_26210) - 5652272..5652586 (+) 315 WP_000349485.1 helix-turn-helix domain-containing protein -
  ABNQ20_RS26220 (ABNQ20_26215) - 5652690..5653166 (-) 477 WP_353629169.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31813.85 Da        Isoelectric Point: 7.7068

>NTDB_id=1009704 ABNQ20_RS26175 WP_003141356.1 5646566..5647438(+) (pilD) [Pseudomonas aeruginosa strain P1110]
MELLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLGDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=1009704 ABNQ20_RS26175 WP_003141356.1 5646566..5647438(+) (pilD) [Pseudomonas aeruginosa strain P1110]
ATGGAACTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAATGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCTGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGGCGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZGZ1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.017

99.655

0.548
  pilD Vibrio campbellii strain DS40M4

53.497

98.621

0.528
  pilD Acinetobacter nosocomialis M2

50.352

97.931

0.493
  pilD Acinetobacter baumannii D1279779

50.352

97.931

0.493
  pilD Neisseria gonorrhoeae MS11

49.281

95.862

0.472

Multiple sequence alignment