Detailed information NTG1009702

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: ABNQ20_RS26165
• Genome accession: NZ_CP158051
• Coordinates: 5643639..5645342 (+)
• Length: 567 a.a.
• NCBI ID: WP_003141354.1
• Uniprot ID: A0A0H2ZH73
• Organism: Pseudomonas aeruginosa strain P1110
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	5642109..5653166	5643639..5645342	within	0

Gene organization within MGE regions

Location: 5642109..5653166

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABNQ20_RS26155 (ABNQ20_26150)	-	5642109..5642666 (-)	558	WP_225011032.1	hypothetical protein	-
ABNQ20_RS26160 (ABNQ20_26155)	-	5642871..5643410 (-)	540	WP_003141352.1	pilin	-
ABNQ20_RS26165 (ABNQ20_26160)	pilB	5643639..5645342 (+)	1704	WP_003141354.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ABNQ20_RS26170 (ABNQ20_26165)	pilC	5645346..5646563 (+)	1218	WP_016254080.1	type II secretion system F family protein	Machinery gene
ABNQ20_RS26175 (ABNQ20_26170)	pilD	5646566..5647438 (+)	873	WP_003141356.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
ABNQ20_RS26180 (ABNQ20_26175)	coaE	5647435..5648046 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
ABNQ20_RS26185 (ABNQ20_26180)	yacG	5648043..5648243 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
ABNQ20_RS26190 (ABNQ20_26185)	-	5648280..5648489 (-)	210	WP_003094660.1	hypothetical protein	-
ABNQ20_RS26195 (ABNQ20_26190)	-	5648595..5649284 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
ABNQ20_RS26200 (ABNQ20_26195)	-	5649281..5649751 (-)	471	WP_003094664.1	hypothetical protein	-
ABNQ20_RS26205 (ABNQ20_26200)	-	5649836..5651113 (-)	1278	WP_000610830.1	ISL3 family transposase	-
ABNQ20_RS26210 (ABNQ20_26205)	-	5651126..5652130 (-)	1005	WP_040363373.1	permease	-
ABNQ20_RS26215 (ABNQ20_26210)	-	5652272..5652586 (+)	315	WP_000349485.1	helix-turn-helix domain-containing protein	-
ABNQ20_RS26220 (ABNQ20_26215)	-	5652690..5653166 (-)	477	WP_353629169.1	GNAT family N-acetyltransferase	-

Sequence

Protein

• Length: 567 a.a.
• Molecular weight: 62610.54 Da
• Isoelectric Point: 5.5603

>NTDB_id=1009702 ABNQ20_RS26165 WP_003141354.1 5643639..5645342(+) (pilB) [Pseudomonas aeruginosa strain P1110]
MNDTIQLSGLARQLVLHELLDEKAAQQAQSQAQRNKLSLVTYLVQSKLVKGQALIELAADQFGIAYCDLNSLERDSLPKD
LISEKLVRQHRVVPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGIIIEKLFENATDSLAGLDDVDLE
GLDVGSGAGASQDDDSSAETDDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFP
EDKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide

• Length: 1704 bp

>NTDB_id=1009702 ABNQ20_RS26165 WP_003141354.1 5643639..5645342(+) (pilB) [Pseudomonas aeruginosa strain P1110]
ATGAACGACACAATTCAACTCAGCGGCTTGGCTCGCCAGCTCGTCCTGCATGAACTTCTCGATGAAAAGGCCGCCCAACA
GGCCCAATCGCAGGCCCAGCGCAACAAGTTGTCGCTGGTAACCTATCTGGTACAGAGCAAGCTAGTGAAAGGGCAGGCCT
TGATCGAACTGGCTGCGGATCAATTCGGTATCGCCTACTGCGACCTGAACAGCCTTGAGCGGGACAGTCTGCCCAAGGAT
CTAATCAGCGAGAAGCTGGTGCGCCAGCATCGAGTAGTTCCGTTGTGGCGACGCGGCAACAAGCTGTTCGTCGGCATCTC
CGATCCAGCCAACCACCAGGCGATTAACGATGTGCAGTTCAGCACAGGCCTGACTACTGAAGCCATCCTGGTCGAGGATG
ACAAGCTCGGCATCATCATCGAGAAACTATTCGAAAATGCCACTGACAGCCTTGCAGGACTGGACGACGTGGATCTGGAA
GGCCTGGATGTCGGCTCCGGTGCGGGCGCAAGCCAGGATGATGATTCCAGTGCAGAGACAGACGACGCCCCAGTGGTGCG
TTTCGTCAACAAAATGTTGTTGGATGCGATCAGAGGCGGCTCCTCCGACCTGCATTTCGAGCCCTATGAAAAGATTTACC
GCGTACGTTTCCGTACCGATGGCATGCTCCATGAAGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGA
CTCAAGGTGATGGCAGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCTA
GCGCGCAGATGGGCATCGACGCCCTGGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCTGCGCTCAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCAC
CGACATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGAC
CTGGAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTGACCCGCCTGCTGAACATGGGCGTGCCAGCGTTCAACCTGGCGACCTCGGTGAATCTGATCATCGCCCAGC
GCCTTGCGCGAAAACTCTGCTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCG
GAAGACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGC
AAGCCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (191-458)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

3D structure

• AlphaFold DB: A0A0H2ZH73

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	57.719	100	0.58
pilB	Acinetobacter baylyi ADP1	57.27	99.471	0.57
pilB	Legionella pneumophila strain ERS1305867	55.106	100	0.552
pilF	Neisseria gonorrhoeae MS11	50.444	99.295	0.501
pilB	Vibrio parahaemolyticus RIMD 2210633	51.948	95.062	0.494
pilB	Vibrio cholerae strain A1552	50.182	97.002	0.487
pilB	Vibrio campbellii strain DS40M4	50.832	95.414	0.485
pilF	Thermus thermophilus HB27	38.584	99.647	0.384
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.189	93.474	0.376