Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ABMW40_RS28125 Genome accession   NZ_CP158049
Coordinates   6043142..6044206 (-) Length   354 a.a.
NCBI ID   WP_282831476.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2-216     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6038142..6049206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABMW40_RS28100 (ABMW40_28085) aroK 6038677..6039195 (-) 519 WP_003095833.1 shikimate kinase AroK -
  ABMW40_RS28105 (ABMW40_28090) pilQ 6039207..6041351 (-) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  ABMW40_RS28110 (ABMW40_28095) pilP 6041405..6041929 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  ABMW40_RS28115 (ABMW40_28100) pilO 6041926..6042549 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  ABMW40_RS28120 (ABMW40_28105) pilN 6042546..6043142 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  ABMW40_RS28125 (ABMW40_28110) pilM 6043142..6044206 (-) 1065 WP_282831476.1 type IV pilus assembly protein PilM Machinery gene
  ABMW40_RS28130 (ABMW40_28115) - 6044391..6046859 (+) 2469 WP_003114576.1 penicillin-binding protein 1A -
  ABMW40_RS28135 (ABMW40_28120) - 6046964..6048232 (-) 1269 WP_003103278.1 malic enzyme -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38013.50 Da        Isoelectric Point: 4.5405

>NTDB_id=1009644 ABMW40_RS28125 WP_282831476.1 6043142..6044206(-) (pilM) [Pseudomonas aeruginosa strain 2-216]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKISTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1009644 ABMW40_RS28125 WP_282831476.1 6043142..6044206(-) (pilM) [Pseudomonas aeruginosa strain 2-216]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCAGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.618

100

0.559
  comM Acinetobacter nosocomialis M2

55.618

100

0.559
  comM Acinetobacter baylyi ADP1

51.695

100

0.517
  pilM Legionella pneumophila strain ERS1305867

46.328

100

0.463

Multiple sequence alignment