Detailed information NTG1009535

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABNG15_RS02005
• Genome accession: NZ_CP158022
• Coordinates: 429737..430885 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain CI00795
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 424737..435885

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABNG15_RS01980 (ABNG15_01980)	-	425888..426184 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ABNG15_RS01985 (ABNG15_01985)	-	426181..426774 (-)	594	WP_367086598.1	YggT family protein	-
ABNG15_RS01990 (ABNG15_01990)	proC	426785..427606 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ABNG15_RS01995 (ABNG15_01995)	-	427618..428310 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ABNG15_RS02000 (ABNG15_02000)	pilT	428525..429559 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ABNG15_RS02005 (ABNG15_02005)	pilU	429737..430885 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
ABNG15_RS02010 (ABNG15_02010)	czcD	430893..431792 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ABNG15_RS02015 (ABNG15_02015)	-	431946..432350 (+)	405	WP_367086599.1	TM2 domain-containing protein	-
ABNG15_RS02020 (ABNG15_02020)	-	432593..433966 (+)	1374	WP_003160915.1	pyridoxal-phosphate dependent enzyme	-
ABNG15_RS02025 (ABNG15_02025)	-	433963..435147 (+)	1185	WP_003117897.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1009535 ABNG15_RS02005 WP_003100959.1 429737..430885(+) (pilU) [Pseudomonas aeruginosa strain CI00795]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1009535 ABNG15_RS02005 WP_003100959.1 429737..430885(+) (pilU) [Pseudomonas aeruginosa strain CI00795]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382