Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q9L42_RS06675 Genome accession   NZ_CP157743
Coordinates   1369700..1370875 (-) Length   391 a.a.
NCBI ID   WP_305909206.1    Uniprot ID   A0AAU7NXS5
Organism   Methylomarinum sp. Ch1-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1364700..1375875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9L42_RS06650 (Q9L42_006650) - 1365511..1365699 (+) 189 WP_305909209.1 DUF2970 domain-containing protein -
  Q9L42_RS06655 (Q9L42_006655) - 1365777..1366178 (-) 402 WP_305909208.1 hypothetical protein -
  Q9L42_RS06660 (Q9L42_006660) - 1366791..1366919 (+) 129 WP_349432386.1 hypothetical protein -
  Q9L42_RS06665 (Q9L42_006665) - 1367518..1369422 (+) 1905 WP_349432388.1 DCC1-like thiol-disulfide oxidoreductase family protein -
  Q9L42_RS06670 (Q9L42_006670) - 1369524..1369646 (+) 123 WP_305909207.1 hypothetical protein -
  Q9L42_RS06675 (Q9L42_006675) pilU 1369700..1370875 (-) 1176 WP_305909206.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q9L42_RS06680 (Q9L42_006680) - 1371181..1372224 (+) 1044 WP_305909205.1 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase -
  Q9L42_RS06685 (Q9L42_006685) - 1372221..1372445 (+) 225 WP_305909204.1 sulfurtransferase TusA family protein -
  Q9L42_RS06690 (Q9L42_006690) - 1372447..1373592 (+) 1146 WP_349432391.1 cation diffusion facilitator family transporter -
  Q9L42_RS06695 (Q9L42_006695) argJ 1373626..1374840 (+) 1215 WP_305909202.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  Q9L42_RS06700 (Q9L42_006700) - 1374821..1375792 (+) 972 WP_349432393.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 43302.38 Da        Isoelectric Point: 5.8938

>NTDB_id=1008102 Q9L42_RS06675 WP_305909206.1 1369700..1370875(-) (pilU) [Methylomarinum sp. Ch1-1]
MEFKDYLKILVHKDGSDLYLTVGAPPAAKFQGALKPLEATALSKERIKAIADDIMDAEQREQFEHVPEMNLAIAEPGIGR
FRVNIFKQRNSHAMVIRNIKVDIPNADDLGLPQILKNKIMEKRGLILFVGGTGSGKSTSLAALIDHRNSHSSGHIITIED
PIEYVHPHKKCLVNQREVGVDTCSYEDALKNTLRQAPDVILIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRGQLLLDLSLNLQAFVSQRLIPTVDGKRTAAIEILLGTQLVSDLIHKGEIHSIKEAMEKSENIGMQTFDSHL
LKLYKAGTISLEEALRNADSPNNLKLKINLSEGRGSATAEETEKSSKSAGLAKLALQELEKDDESEEENEK

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=1008102 Q9L42_RS06675 WP_305909206.1 1369700..1370875(-) (pilU) [Methylomarinum sp. Ch1-1]
ATGGAATTCAAAGATTACCTTAAGATTCTTGTGCATAAAGATGGTTCCGACCTTTACCTGACCGTCGGCGCGCCGCCGGC
GGCGAAATTTCAGGGCGCCCTAAAACCGCTGGAGGCGACGGCGCTGAGCAAGGAGCGAATCAAGGCGATTGCCGACGACA
TCATGGATGCCGAACAGCGCGAGCAATTTGAGCATGTTCCGGAAATGAACCTGGCCATCGCCGAGCCAGGCATCGGCCGG
TTTCGGGTTAATATCTTCAAACAACGCAACAGCCATGCGATGGTGATACGCAACATCAAGGTAGACATCCCCAACGCCGA
TGACCTAGGCCTGCCGCAGATACTGAAAAACAAGATTATGGAAAAACGCGGCTTGATCCTGTTCGTCGGCGGCACCGGTT
CCGGGAAATCGACCTCGCTGGCCGCGCTGATCGACCATCGCAACAGCCATTCATCGGGCCATATCATCACGATAGAAGAT
CCGATTGAATATGTTCATCCGCATAAAAAATGCCTGGTCAACCAACGGGAAGTCGGCGTCGACACCTGCTCCTACGAAGA
CGCGCTGAAAAACACCTTGAGACAGGCCCCGGACGTCATCTTGATTGGCGAGATCCGCAGCCAGGAAACCATGGAGCACG
CACTGGCCTTCGCCGAAACCGGTCACTTATGCCTTTCGACGCTGCATGCCAACAACGCCAACCAGGCCTTAGACAGAATC
ATCAATTTCTTTCCGGAAGAACGCCGCGGCCAATTATTGCTGGACTTATCACTGAACCTACAGGCATTTGTCTCGCAAAG
GCTAATCCCGACCGTCGACGGCAAAAGAACCGCAGCAATCGAAATCCTGCTGGGCACACAGTTGGTCAGCGACCTGATTC
ATAAAGGCGAAATCCATTCCATCAAGGAGGCGATGGAAAAATCGGAAAACATCGGCATGCAAACCTTCGATAGCCATCTG
CTAAAACTCTACAAAGCCGGAACCATTTCTCTGGAAGAAGCGCTCCGCAATGCGGATTCGCCAAACAACCTGAAATTAAA
AATCAACCTGAGTGAAGGACGTGGCTCAGCGACAGCAGAAGAGACCGAAAAATCAAGTAAATCGGCCGGCTTGGCTAAAC
TGGCTTTACAGGAACTGGAAAAGGATGACGAAAGCGAGGAGGAGAATGAAAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(45-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.25

98.21

0.552
  pilU Acinetobacter baylyi ADP1

56.215

90.537

0.509
  pilU Vibrio cholerae strain A1552

50.847

90.537

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.556

92.072

0.373

Multiple sequence alignment