Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ABLV49_RS19715 Genome accession   NZ_CP157675
Coordinates   4117172..4117693 (-) Length   173 a.a.
NCBI ID   WP_349279140.1    Uniprot ID   A0AAU7LRJ1
Organism   Polaromonas hydrogenivorans strain DSM 17735     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4112172..4122693
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABLV49_RS19695 (ABLV49_19695) - 4112332..4113915 (-) 1584 WP_349281819.1 M48 family metalloprotease -
  ABLV49_RS19700 (ABLV49_19700) moaC 4114056..4114586 (+) 531 WP_349279135.1 cyclic pyranopterin monophosphate synthase MoaC -
  ABLV49_RS19705 (ABLV49_19705) - 4114608..4116143 (-) 1536 WP_349279137.1 Wzy polymerase domain-containing protein -
  ABLV49_RS19710 (ABLV49_19710) tfpZ 4116321..4117088 (-) 768 WP_349279138.1 TfpX/TfpZ family type IV pilin accessory protein -
  ABLV49_RS19715 (ABLV49_19715) pilA 4117172..4117693 (-) 522 WP_349279140.1 pilin Machinery gene
  ABLV49_RS19720 (ABLV49_19720) - 4118069..4118635 (-) 567 WP_349279142.1 FHA domain-containing protein -
  ABLV49_RS19725 (ABLV49_19725) - 4118675..4119460 (-) 786 WP_349279144.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  ABLV49_RS19730 (ABLV49_19730) - 4119614..4120315 (-) 702 WP_349279146.1 TerC family protein -
  ABLV49_RS19735 (ABLV49_19735) sucD 4120430..4121326 (-) 897 WP_349279148.1 succinate--CoA ligase subunit alpha -
  ABLV49_RS19740 (ABLV49_19740) sucC 4121344..4122504 (-) 1161 WP_349279150.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 17837.88 Da        Isoelectric Point: 10.0653

>NTDB_id=1008058 ABLV49_RS19715 WP_349279140.1 4117172..4117693(-) (pilA) [Polaromonas hydrogenivorans strain DSM 17735]
MKRSMQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARTTEGLSLAAAAKLNVAEFAARGADVAVATGYNTGFNVVPF
VPTANVLNLTIAPATGVISINYTTRVQVAATNLLTLNPYTGTQAAPALLPVGTGIFTPPAEPVKWRCRAAGAVGTLIGTA
GTLPARLAPGECR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=1008058 ABLV49_RS19715 WP_349279140.1 4117172..4117693(-) (pilA) [Polaromonas hydrogenivorans strain DSM 17735]
ATGAAGCGTTCCATGCAAAAGGGTTTCACCCTGATCGAATTGATGATCGTTGTGGCGATTATTGGTATTTTGGCTGCCGT
GGCTTTGCCTGCTTACCAGGATTACACGATTCGTGCCCGCACAACTGAAGGGTTGTCTTTGGCTGCTGCTGCAAAGCTTA
ATGTTGCTGAGTTTGCAGCACGTGGTGCGGATGTAGCTGTAGCTACCGGTTACAACACGGGATTTAATGTTGTGCCTTTT
GTACCGACGGCCAATGTACTGAATTTGACTATTGCTCCTGCTACAGGCGTAATTTCCATTAATTACACAACGCGTGTCCA
AGTTGCGGCGACGAATTTGCTCACTCTCAATCCTTACACCGGAACGCAAGCCGCTCCTGCGCTGCTGCCGGTAGGCACTG
GCATTTTTACCCCACCAGCAGAACCAGTTAAATGGCGTTGCCGCGCCGCTGGCGCTGTTGGTACGCTGATTGGCACTGCT
GGCACGCTGCCCGCAAGGCTTGCACCAGGCGAATGTCGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-29)

Pilin (bacterial filament) (Pilin)

(38-173)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

46.961

100

0.491
  pilA2 Legionella pneumophila strain ERS1305867

45.614

98.844

0.451
  pilA2 Legionella pneumophila str. Paris

45.029

98.844

0.445
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.025

100

0.399

Multiple sequence alignment