Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ABL850_RS26045 Genome accession   NZ_CP157628
Coordinates   5439758..5440222 (-) Length   154 a.a.
NCBI ID   WP_049809881.1    Uniprot ID   -
Organism   Variovorax paradoxus strain 369MFTsu5.1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5434758..5445222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL850_RS26025 (ABL850_26005) - 5435110..5435595 (-) 486 WP_018903505.1 hypothetical protein -
  ABL850_RS26030 (ABL850_26010) - 5435714..5437339 (-) 1626 WP_418146301.1 NAD(P)/FAD-dependent oxidoreductase -
  ABL850_RS26035 (ABL850_26015) - 5437350..5437787 (-) 438 WP_049809883.1 GtrA family protein -
  ABL850_RS26040 (ABL850_26020) - 5437777..5439693 (-) 1917 WP_027730002.1 hypothetical protein -
  ABL850_RS26045 (ABL850_26025) pilA 5439758..5440222 (-) 465 WP_049809881.1 pilin Machinery gene
  ABL850_RS26050 (ABL850_26030) - 5440428..5441231 (-) 804 WP_027730000.1 MBL fold metallo-hydrolase -
  ABL850_RS26055 (ABL850_26035) - 5441264..5443540 (-) 2277 WP_027729999.1 CHASE2 domain-containing protein -
  ABL850_RS26060 (ABL850_26040) - 5443583..5444239 (-) 657 WP_018903498.1 FHA domain-containing protein -
  ABL850_RS26065 (ABL850_26045) - 5444264..5445064 (-) 801 WP_018903497.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 15769.18 Da        Isoelectric Point: 9.6044

>NTDB_id=1007846 ABL850_RS26045 WP_049809881.1 5439758..5440222(-) (pilA) [Variovorax paradoxus strain 369MFTsu5.1]
MNVRKIARRAQAGFTLIELMIVVAIIGILAAVALPAYQDYTVRARVSEAMVLASAAKTLVGENAAAAASSLTLGFDSASV
SSTKNVASMDIGSAGAISVVTTAAAGGVTLVLTPTSDGQALSANTPPTKQIVWKCTTSDTTKFKYVPSECRQTS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=1007846 ABL850_RS26045 WP_049809881.1 5439758..5440222(-) (pilA) [Variovorax paradoxus strain 369MFTsu5.1]
ATGAACGTTCGCAAGATCGCACGCCGCGCACAGGCCGGCTTTACCCTTATCGAATTGATGATCGTTGTGGCGATCATTGG
TATCTTGGCTGCAGTGGCTCTGCCGGCGTATCAGGACTACACCGTGCGCGCACGTGTGTCGGAAGCCATGGTGCTCGCAT
CGGCGGCCAAGACGCTGGTCGGTGAAAACGCAGCTGCTGCGGCCTCCAGCCTCACCCTCGGCTTTGATTCTGCTTCCGTT
TCGTCCACCAAGAACGTCGCCAGCATGGATATCGGCTCTGCAGGCGCTATTTCCGTTGTTACGACTGCGGCTGCGGGTGG
TGTTACGTTGGTTTTGACGCCCACTTCCGATGGGCAGGCGCTGAGCGCCAATACGCCTCCGACCAAGCAGATCGTGTGGA
AGTGCACAACTTCGGACACTACCAAGTTCAAGTACGTTCCGAGCGAGTGCCGCCAAACCTCCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-34)

Pilin (bacterial filament) (Pilin)

(42-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

48.81

100

0.532
  comP Acinetobacter baylyi ADP1

50.318

100

0.513
  pilA2 Legionella pneumophila str. Paris

52.414

94.156

0.494
  pilA2 Legionella pneumophila strain ERS1305867

51.034

94.156

0.481

Multiple sequence alignment