Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ABLB99_RS01490 Genome accession   NZ_CP157447
Coordinates   319519..320259 (+) Length   246 a.a.
NCBI ID   WP_006581061.1    Uniprot ID   A0A8B4N9D4
Organism   Acinetobacter nosocomialis strain XH1727     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 314519..325259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABLB99_RS01475 (ABLB99_01475) ponA 315100..317658 (-) 2559 WP_004884735.1 penicillin-binding protein PBP1a -
  ABLB99_RS01480 (ABLB99_01480) comM 317820..318878 (+) 1059 WP_004711654.1 pilus assembly protein PilM Machinery gene
  ABLB99_RS01485 (ABLB99_01485) pilN 318878..319522 (+) 645 WP_006581060.1 PilN domain-containing protein Machinery gene
  ABLB99_RS01490 (ABLB99_01490) pilO 319519..320259 (+) 741 WP_006581061.1 type 4a pilus biogenesis protein PilO Machinery gene
  ABLB99_RS01495 (ABLB99_01495) pilP 320270..320797 (+) 528 WP_004711660.1 pilus assembly protein PilP Machinery gene
  ABLB99_RS01500 (ABLB99_01500) pilQ 320860..323025 (+) 2166 WP_004711661.1 type IV pilus secretin PilQ family protein Machinery gene
  ABLB99_RS01505 (ABLB99_01505) aroK 323037..323606 (+) 570 WP_002051689.1 shikimate kinase AroK -
  ABLB99_RS01510 (ABLB99_01510) aroB 323635..324717 (+) 1083 WP_002051763.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27611.37 Da        Isoelectric Point: 4.9673

>NTDB_id=1006911 ABLB99_RS01490 WP_006581061.1 319519..320259(+) (pilO) [Acinetobacter nosocomialis strain XH1727]
MSQDELQELSLEQSASKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
GNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSISALPRIVTMHDFVVEAAQAKDSKSDIPVLNYSIKAKTYRYIGATEASAQPAKAAAATAPTS
PNAQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=1006911 ABLB99_RS01490 WP_006581061.1 319519..320259(+) (pilO) [Acinetobacter nosocomialis strain XH1727]
ATGAGTCAAGATGAACTGCAAGAATTATCATTAGAGCAATCAGCTTCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTTGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACTTGCTGGATTTTTATTT
TCTTTGCTGTATTGGCTTTGGGTTATTTTGTTGCTATTCAGCCAAAGCTACAAGCAATTGATAATGCCCAAGCTCAGGAA
GGTAATTTATTAAATGAATTTCGAGAAAAAGACTCCAAATTACGTAACCTTCAGCAGTATCAAGTGCAGCTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAATTAGAACAGTTGCCGAAAGAAACAGAGATTCCAAGTTTAGTTGAGGATATTAACTTAA
CTGGCGTGAACTCTGGATTGAAATTTAAAAATATTCGTTTAGAGGATGAAGTAAAGCAGGAAATTTTTATTGAACAACCA
ATTGCCATCGAAGCAACAGGCGACTATCACGCTTTCGGTGCTTTCGTAAGTAGTATTTCTGCTTTGCCACGTATTGTGAC
GATGCATGACTTTGTTGTAGAGGCTGCTCAAGCTAAAGATAGTAAGTCAGATATTCCTGTGCTTAATTATTCTATTAAAG
CCAAAACCTATCGATATATAGGAGCAACAGAGGCATCGGCACAACCAGCTAAGGCTGCAGCGGCGACTGCACCAACTAGC
CCTAATGCTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A8B4N9D4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

89.431

100

0.894
  comO Acinetobacter baylyi ADP1

70.954

97.967

0.695

Multiple sequence alignment