Detailed information NTG1006800

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABLC00_RS04530
• Genome accession: NZ_CP157432
• Coordinates: 948617..949735 (-)
• Length: 372 a.a.
• NCBI ID: WP_002052019.1
• Uniprot ID: A0A1V0YUM2
• Organism: Acinetobacter nosocomialis strain XH1679
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 943617..954735

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABLC00_RS04500 (ABLC00_04500)	-	944445..945293 (+)	849	WP_022575107.1	class II glutamine amidotransferase	-
ABLC00_RS04505 (ABLC00_04505)	-	945546..946001 (+)	456	WP_000782976.1	bacteriohemerythrin	-
ABLC00_RS04510 (ABLC00_04510)	-	946154..947221 (+)	1068	WP_033846680.1	membrane protein	-
ABLC00_RS04515 (ABLC00_04515)	-	947221..947544 (+)	324	WP_002052062.1	RnfH family protein	-
ABLC00_RS04520 (ABLC00_04520)	-	947600..947998 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
ABLC00_RS04525 (ABLC00_04525)	fur	948110..948547 (+)	438	WP_002052029.1	ferric iron uptake transcriptional regulator	-
ABLC00_RS04530 (ABLC00_04530)	pilU	948617..949735 (-)	1119	WP_002052019.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ABLC00_RS04535 (ABLC00_04535)	pilT	949763..950800 (-)	1038	WP_006581387.1	type IV pilus twitching motility protein PilT	Machinery gene
ABLC00_RS04540 (ABLC00_04540)	-	950927..951619 (+)	693	WP_032010894.1	YggS family pyridoxal phosphate-dependent enzyme	-
ABLC00_RS04545 (ABLC00_04545)	-	951727..952569 (+)	843	WP_033846643.1	undecaprenyl-diphosphate phosphatase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41980.18 Da
• Isoelectric Point: 6.6944

>NTDB_id=1006800 ABLC00_RS04530 WP_002052019.1 948617..949735(-) (pilU) [Acinetobacter nosocomialis strain XH1679]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPTVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLANEGPDQITDEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=1006800 ABLC00_RS04530 WP_002052019.1 948617..949735(-) (pilU) [Acinetobacter nosocomialis strain XH1679]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGTGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGCCTTTCAGGTGAAGTGATTGGACAGCTCTTACATTCCA
TCATGAGTGAAAAACAACGTAAAGAATTTGCAGAGACTCGTGAATGTAACTTTGCCATCATGAACCGCGATAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAACGTGACATGCCGGGTATGGTACTACGTCGAATTGAAACGAAAATTCCTTCAAT
TGATGACTTACAGTTGCCGACTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGTGCAACAG
GTACAGGTAAATCGACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAG
GACCCGATTGAGTTTATTCACGAACATGCGGGCTGCATTATTACTCAACGTGAAGTCGGTATTGATACGGACTCATTCGA
AATTGCGTTAAAAAACACGTTACGACAAGCGCCAGATGTGATCTTAATTGGTGAAATCCGCTCTCGTGAAGTCATGGACT
ACGCAATTGGCTTTGCTGAAACAGGACACCTTGTATTAGCTACAATGCATGCCAACAATGCCAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTTTACATGGACTTGTCTCTTAACTTAAAAGCCATGATTGCACA
ACAGCTTATTCCAACACCAGATGGTAATTCACGCCGTGCAGCGATTGAGATCTTAATTAACTCACCATTAATTTCAGACT
ATATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTGATGAAACGCTCACGTGAGCTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATACAAAGCAGGCCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTAACGATTAAACTTGCAAATGAAGGGCCTGATCAAATTACAGACGAAAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A1V0YUM2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.254	95.43	0.403