Detailed information NTG1006227

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ABHN08_RS26155
• Genome accession: NZ_CP157354
• Coordinates: 5756594..5757658 (+)
• Length: 354 a.a.
• NCBI ID: WP_024011266.1
• Uniprot ID: V8RDF4
• Organism: Pseudomonas atacamensis strain GY-AP11
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5751594..5762658

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABHN08_RS26145 (ABHN08_26145)	-	5752341..5753609 (+)	1269	WP_016772053.1	malic enzyme-like NAD(P)-binding protein	-
ABHN08_RS26150 (ABHN08_26150)	-	5753930..5756374 (-)	2445	WP_419096399.1	penicillin-binding protein 1A	-
ABHN08_RS26155 (ABHN08_26155)	pilM	5756594..5757658 (+)	1065	WP_024011266.1	pilus assembly protein PilM	Machinery gene
ABHN08_RS26160 (ABHN08_26160)	-	5757658..5758224 (+)	567	WP_016772051.1	PilN domain-containing protein	-
ABHN08_RS26165 (ABHN08_26165)	pilO	5758221..5758844 (+)	624	WP_071174143.1	type 4a pilus biogenesis protein PilO	-
ABHN08_RS26170 (ABHN08_26170)	-	5758841..5759365 (+)	525	WP_071174144.1	pilus assembly protein PilP	-
ABHN08_RS26175 (ABHN08_26175)	pilQ	5759379..5761457 (+)	2079	WP_071174145.1	type IV pilus secretin PilQ	Machinery gene
ABHN08_RS26180 (ABHN08_26180)	aroK	5761462..5761980 (+)	519	WP_024011270.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38045.57 Da
• Isoelectric Point: 4.7539

>NTDB_id=1006227 ABHN08_RS26155 WP_024011266.1 5756594..5757658(+) (pilM) [Pseudomonas atacamensis strain GY-AP11]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIAELEGVGQALSRLLVKARTGVKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATHLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLDTPTQVANP
FADMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1006227 ABHN08_RS26155 WP_024011266.1 5756594..5757658(+) (pilM) [Pseudomonas atacamensis strain GY-AP11]
GTGCTGGGACTCTTCAATAAAAAGACCAATGCGTTACTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTGGAACT
GAGCCGTCAGGGCGAGCGCTACCGGGTCGAGGCCTATGCGGTGGAGCCGTTGCCGGCGAACGCTGTGGTCGAAAAGAACA
TCGCCGAACTCGAGGGCGTTGGCCAGGCACTGAGCCGCCTGCTGGTCAAGGCGCGTACCGGGGTCAAGAGCGTGGCAGTG
GCGGTGGCCGGTTCGGCGGTGATCACCAAGGTCATCGAGATGGACGCCGGGCTGTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAGGCCGACCAATACATTCCCTATCCGCTGGACGAAGTCGCCATCGACTTTGAAGTCCAGGGCGTCTCGCCGC
GCAACCCGGAACGGGTCAACGTGCTGCTCGCCGCCTGTCGCAAGGAAAACGTCGAGGTGCGTGAAGCGGCGCTGGCGCTC
GCCGGCCTGACCGCGCGGGTGGTGGATGTCGAGGCCTACGCGCTGGAGCGCTCGTTCGGCCTGCTGGCGACGCATCTGGC
GGCCTCCCAGGAGCGCCTGACCGTGGCAGTGGTCGACATCGGTGCGACCATGACCACCCTCAGCGTCCTGCACAACGGCA
AGATCATCTACACCCGCGAGCAATTGTTCGGCGGACGCCAGCTCACCGAAGAGATCCAGCGCCGCTACGGCCTCACGGTC
GAACAGGCGGGCCTGGCGAAAAAACAGGGCGGGCTGCCTGACGATTACGTCAGCGAGGTCCTGCAGCCATTTCGCGAGGC
GCTGGTGCAGCAGGTCTCGCGCTCGCTGCAGTTCTTCTTCGCCTCCGGCCAGTACAACGCGGTCGACCATATCCTGCTGG
CCGGCGGCACCGCCTCGGTGCCCGGCCTGGACCGTCTGATCGAACAGCGGCTGGACACGCCGACGCAGGTCGCCAACCCG
TTCGCCGACATGGCCTTGGGCAGCAAGGTCAATGCTGGGGCGCTGGCCAGTGATGCGCCAGCCTTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: V8RDF4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	54.52	100	0.545
pilM	Legionella pneumophila strain ERS1305867	48.305	100	0.483