Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   ABKA22_RS05545 Genome accession   NZ_CP157287
Coordinates   1089131..1090453 (-) Length   440 a.a.
NCBI ID   WP_348619229.1    Uniprot ID   -
Organism   Lactococcus lactis strain BIM B-1834     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1084131..1095453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABKA22_RS05515 (ABKA22_05515) - 1085289..1086242 (+) 954 WP_023189344.1 PhoH family protein -
  ABKA22_RS05520 (ABKA22_05520) - 1086300..1086779 (+) 480 WP_003130650.1 NUDIX hydrolase -
  ABKA22_RS05525 (ABKA22_05525) ybeY 1086931..1087419 (+) 489 WP_003130652.1 rRNA maturation RNase YbeY -
  ABKA22_RS05530 (ABKA22_05530) - 1087403..1087858 (+) 456 WP_003130653.1 diacylglycerol kinase -
  ABKA22_RS05535 (ABKA22_05535) - 1087872..1088450 (+) 579 WP_003130655.1 nucleotidyltransferase family protein -
  ABKA22_RS05540 (ABKA22_05540) comFC 1088484..1088888 (-) 405 WP_003130657.1 ComF family protein Machinery gene
  ABKA22_RS05545 (ABKA22_05545) comFA 1089131..1090453 (-) 1323 WP_348619229.1 DEAD/DEAH box helicase Machinery gene
  ABKA22_RS05550 (ABKA22_05550) - 1090510..1091139 (+) 630 WP_003130660.1 YigZ family protein -
  ABKA22_RS05555 (ABKA22_05555) - 1091273..1091770 (+) 498 WP_003130662.1 VanZ family protein -
  ABKA22_RS05560 (ABKA22_05560) - 1091818..1092837 (+) 1020 WP_003130663.1 DUF475 domain-containing protein -
  ABKA22_RS05565 (ABKA22_05565) - 1092952..1093698 (+) 747 WP_348619230.1 bifunctional metallophosphatase/5'-nucleotidase -
  ABKA22_RS05570 (ABKA22_05570) glyQ 1093983..1094936 (+) 954 WP_003130667.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50216.81 Da        Isoelectric Point: 10.1065

>NTDB_id=1005433 ABKA22_RS05545 WP_348619229.1 1089131..1090453(-) (comFA) [Lactococcus lactis strain BIM B-1834]
MSTNQEKLFGRLLLKNDILQLIKNTDKISVSKIFSNFLLETKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYV
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHIFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERLTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=1005433 ABKA22_RS05545 WP_348619229.1 1089131..1090453(-) (comFA) [Lactococcus lactis strain BIM B-1834]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTGCAACTTATAAAAAATACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAACGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGCTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCTGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTTTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATGTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACATTTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACATCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCTAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(114-233)

Helicase conserved C-terminal domain (Helicase_C)

(300-403)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74.25

90.909

0.675
  comFA/cflA Streptococcus pneumoniae Rx1

52.261

90.455

0.473
  comFA/cflA Streptococcus pneumoniae D39

52.261

90.455

0.473
  comFA/cflA Streptococcus pneumoniae R6

52.261

90.455

0.473
  comFA/cflA Streptococcus pneumoniae TIGR4

52.261

90.455

0.473
  comFA/cflA Streptococcus mitis SK321

51.759

90.455

0.468
  comFA/cflA Streptococcus mitis NCTC 12261

51.005

90.455

0.461
  comFA Bacillus subtilis subsp. subtilis str. 168

38.018

98.636

0.375

Multiple sequence alignment