Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   E0F34_RS01440 Genome accession   NZ_LR536843
Coordinates   247519..248517 (+) Length   332 a.a.
NCBI ID   WP_001809468.1    Uniprot ID   Q8CWU7
Organism   Streptococcus pneumoniae strain GPSC55 substr. ST3774 isolate b04a6400-1f66-11e7-b93e-3c4a9275d6c8     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 242519..253517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F34_RS01420 (SAMEA104035599_00270) - 244467..245309 (+) 843 WP_001081167.1 hypothetical protein -
  E0F34_RS01425 (SAMEA104035599_00271) - 245365..246060 (+) 696 WP_001144904.1 GNAT family N-acetyltransferase -
  E0F34_RS01430 (SAMEA104035599_00272) - 246072..246488 (+) 417 WP_061390004.1 GNAT family N-acetyltransferase -
  E0F34_RS01435 - 247293..247385 (-) 93 WP_000970381.1 type I toxin-antitoxin system Fst family toxin -
  E0F34_RS01440 (SAMEA104035599_00273) ruvB 247519..248517 (+) 999 WP_001809468.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  E0F34_RS01445 (SAMEA104035599_00274) - 248555..249070 (+) 516 WP_232036072.1 nucleotidyltransferase family protein -
  E0F34_RS01450 (SAMEA104035599_00275) - 249301..250059 (+) 759 WP_000466719.1 isoprenyl transferase -
  E0F34_RS01455 (SAMEA104035599_00276) - 250068..250871 (+) 804 WP_050147872.1 phosphatidate cytidylyltransferase -
  E0F34_RS01460 (SAMEA104035599_00277) eeP 250893..252152 (+) 1260 WP_000900660.1 RIP metalloprotease RseP Regulator

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 37288.73 Da        Isoelectric Point: 4.5053

>NTDB_id=1004432 E0F34_RS01440 WP_001809468.1 247519..248517(+) (ruvB) [Streptococcus pneumoniae strain GPSC55 substr. ST3774 isolate b04a6400-1f66-11e7-b93e-3c4a9275d6c8]
MSRILDNEIMGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPVIEKAGDLVAILNELEPGDVLFIDEIHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRVRDFAQIMGNGVID
DIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSVNIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAK
AYEHLGYEYSEK

Nucleotide


Download         Length: 999 bp        

>NTDB_id=1004432 E0F34_RS01440 WP_001809468.1 247519..248517(+) (ruvB) [Streptococcus pneumoniae strain GPSC55 substr. ST3774 isolate b04a6400-1f66-11e7-b93e-3c4a9275d6c8]
ATGAGTAGAATTTTAGATAATGAGATAATGGGGGATGAGGAGTTAGTAGAACGCACGCTCCGTCCTCAGTATTTACGTGA
ATATATCGGACAGGATAAGGTCAAGGACCAGCTACAAATCTTTATTGAAGCTGCCAAAATGCGGGATGAAGCGCTGGATC
ATGTGCTCTTATTTGGGCCTCCAGGTCTCGGGAAAACGACCATGGCCTTTGTTATTGCCAACGAACTGGGAGTCAATCTC
AAGCAGACGTCGGGTCCAGTCATTGAAAAAGCCGGAGATCTGGTAGCTATTTTGAATGAGTTAGAGCCTGGGGATGTACT
TTTTATTGATGAGATCCATCGTTTGCCAATGTCAGTGGAAGAGGTGCTTTATAGTGCTATGGAGGACTTCTACATCGATA
TTATGATTGGGGCTGGTGAGGGTAGTCGTAGTGTTCATTTGGAGTTACCACCTTTTACCTTGATTGGTGCGACGACTCGG
GCTGGTATGCTCTCCAATCCGCTACGGGCACGTTTTGGGATTACAGGCCATATGGAGTATTATGCCCATGCTGACTTGAC
AGAAATTGTCGAGCGGACGGCAGATATTTTTGAGATGGAAATCACTCATGAGGCAGCATCTGAGTTGGCCCTACGTAGTC
GTGGGACCCCTCGTATTGCCAATCGTCTCCTCAAGCGCGTGCGCGATTTTGCCCAGATAATGGGGAATGGGGTAATTGAT
GATATTATTACCGATAAGGCTTTGACTATGCTGGATGTTGACCATGAAGGTTTGGACTATGTGGATCAAAAAATCCTTCG
TACCATGATTGAGATGTACAGTGGAGGACCTGTTGGTCTAGGAACTCTTTCTGTGAATATCGCCGAAGAGCGTGAGACAG
TTGAAGACATGTATGAGCCTTACTTGATTCAAAAAGGTTTTATCATGCGGACACGGTCTGGACGGGTGGCGACTGCTAAG
GCATATGAGCACTTAGGTTATGAATACAGTGAAAAATAA

Domains

Predicted by InterProScan.

RuvB AAA lid domain (AAA_lid_4)

(181-254)

RuvB C-terminal winged helix domain (WHD_RuvB)

(256-325)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(20-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8CWU7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Streptococcus pneumoniae R6

100

100

1
  ruvB Streptococcus pneumoniae D39

100

100

1
  ruvB Streptococcus pneumoniae TIGR4

99.096

100

0.991
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.429

98.193

0.593
  ruvB Helicobacter pylori 26695

50.774

97.289

0.494
  ruvB Synechocystis sp. PCC 6803

49.84

94.277

0.47