Detailed information NTG1003827

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABH008_RS05325
• Genome accession: NZ_CP157005
• Coordinates: 1160460..1161605 (+)
• Length: 381 a.a.
• NCBI ID: WP_347988816.1
• Uniprot ID: -
• Organism: Methylomonas sp. AM2-LC
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1155460..1166605

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABH008_RS05305	-	1155760..1156029 (-)	270	WP_347989948.1	septum formation initiator family protein	-
ABH008_RS05310	eno	1156143..1157426 (-)	1284	WP_347988813.1	phosphopyruvate hydratase	-
ABH008_RS05315	kdsA	1157439..1158272 (-)	834	WP_347988814.1	3-deoxy-8-phosphooctulonate synthase	-
ABH008_RS05320	-	1158275..1159903 (-)	1629	WP_347988815.1	CTP synthase	-
ABH008_RS05325	pilU	1160460..1161605 (+)	1146	WP_347988816.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ABH008_RS05330	-	1161772..1163001 (-)	1230	WP_347988817.1	pyrophosphate--fructose-6-phosphate 1-phosphotransferase	-
ABH008_RS05335	-	1163229..1164023 (+)	795	WP_347988818.1	DUF2797 domain-containing protein	-
ABH008_RS05340	-	1164020..1164277 (-)	258	WP_347988819.1	hypothetical protein	-
ABH008_RS05345	-	1164422..1165198 (-)	777	WP_347988820.1	alpha/beta fold hydrolase	-
ABH008_RS05350	-	1165230..1165832 (-)	603	WP_347988821.1	arylesterase	-
ABH008_RS05355	-	1165834..1166523 (+)	690	WP_347989949.1	ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42427.82 Da
• Isoelectric Point: 6.8402

>NTDB_id=1003827 ABH008_RS05325 WP_347988816.1 1160460..1161605(+) (pilU) [Methylomonas sp. AM2-LC]
MELKDYLKILVQNDGSDLYLTTNAPPAGKFHGVLKPLENIKLTSQRIKEIADSIMDAEQRRSFESIPEMNLAIAEEGIGR
FRVNIYKQRNDYALVIRNIKVTIPNVDELGLPEVLKRKIMEKRGLILFVGGTGSGKSTSLAALLDYRNSNSSGHIITIED
PIEYIHPHKKSLVSQREVGVDTLSYADALKNTLRQAPDVIQIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRNQLLMDLSLNLQAFVSQRLVPTLDGKRVAAIEILLGTKLVSDLIFKGDVHGIKEVMEKSVEVGMQTFDYHL
LQLYKSGIISLDNALQNSDSPNNLKLKINLSEGKGSVGGLQIENIKKLTSSLSLHPILKDE

Nucleotide

• Length: 1146 bp

>NTDB_id=1003827 ABH008_RS05325 WP_347988816.1 1160460..1161605(+) (pilU) [Methylomonas sp. AM2-LC]
ATGGAATTAAAAGACTATTTAAAAATATTGGTACAGAATGATGGTTCTGATCTATATCTAACCACAAATGCACCCCCTGC
TGGAAAATTTCATGGCGTTCTAAAGCCATTGGAAAACATTAAGCTTACCAGTCAACGCATCAAAGAAATTGCTGATAGCA
TTATGGATGCTGAACAACGCAGGAGTTTTGAAAGCATACCAGAAATGAATCTGGCTATAGCTGAAGAAGGGATAGGGCGT
TTCAGGGTAAATATTTACAAACAGCGCAATGACTATGCTTTGGTTATTCGTAACATCAAGGTAACTATTCCTAATGTTGA
TGAATTGGGCTTGCCCGAAGTTCTTAAGCGTAAAATTATGGAGAAACGCGGTCTGATATTATTTGTGGGTGGTACGGGTT
CAGGGAAATCTACTTCTCTGGCTGCACTACTTGATTATCGAAACAGTAATTCGTCTGGACACATAATTACCATTGAAGAT
CCGATAGAGTATATACACCCGCATAAAAAATCGTTGGTCAGTCAACGCGAGGTTGGTGTGGATACCCTGAGCTATGCTGA
CGCATTAAAAAATACTTTACGCCAAGCACCTGATGTCATCCAAATCGGCGAAATTCGTAGTCAGGAAACCATGGAGCATG
CATTGGCTTTCGCGGAAACCGGACATTTGTGTTTATCAACTCTACACGCCAATAATGCGAATCAGGCTCTGGATCGGATT
ATCAATTTTTTTCCAGAAGAGCGGCGTAATCAATTATTAATGGATTTGTCCTTGAATCTTCAAGCGTTTGTGTCCCAGCG
CTTAGTGCCGACTCTGGATGGTAAGCGTGTAGCTGCTATCGAGATTTTGTTAGGGACTAAGTTGGTGAGTGACTTAATCT
TTAAAGGTGATGTACATGGTATTAAGGAAGTTATGGAAAAGTCAGTAGAAGTGGGTATGCAGACTTTCGATTATCATCTA
CTGCAATTGTATAAATCAGGAATCATTTCATTGGATAATGCCTTACAAAACTCCGATTCCCCAAATAACTTGAAATTGAA
AATTAATCTTAGTGAAGGAAAAGGTTCTGTAGGTGGATTGCAAATAGAAAATATTAAAAAATTGACTAGCAGTCTATCAT
TGCATCCAATACTCAAAGATGAATAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (44-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.583	93.701	0.53
pilU	Acinetobacter baylyi ADP1	53.107	92.913	0.493
pilU	Vibrio cholerae strain A1552	47.956	96.325	0.462
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.111	94.226	0.378