Detailed information NTG1003534

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EL297_RS11420
• Genome accession: NZ_LR134525
• Coordinates: 1922446..1923672 (-)
• Length: 408 a.a.
• NCBI ID: WP_134990390.1
• Uniprot ID: -
• Organism: Neisseria meningitidis strain NCTC10025
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1917446..1928672

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL297_RS11395 (NCTC10025_01915)	-	1917447..1918454 (+)	1008	WP_134990389.1	IS5 family transposase	-
EL297_RS11410 (NCTC10025_01916)	yccS	1920010..1922160 (-)	2151	WP_082308663.1	YccS family putative transporter	-
EL297_RS11420 (NCTC10025_01917)	pilU	1922446..1923672 (-)	1227	WP_134990390.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EL297_RS11425 (NCTC10025_01918)	pilT	1923835..1924872 (-)	1038	WP_226893076.1	type IV pilus twitching motility protein PilT	Machinery gene
EL297_RS11430 (NCTC10025_01919)	-	1924998..1925684 (+)	687	WP_002239404.1	YggS family pyridoxal phosphate-dependent enzyme	-
EL297_RS11435 (NCTC10025_01920)	-	1925684..1926091 (+)	408	WP_002239400.1	hypothetical protein	-
EL297_RS11440 (NCTC10025_01921)	proC	1926122..1926931 (+)	810	WP_226893052.1	pyrroline-5-carboxylate reductase	-
EL297_RS11450 (NCTC10025_01922)	dksA	1927079..1927495 (+)	417	WP_002231430.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45673.29 Da
• Isoelectric Point: 7.1275

>NTDB_id=1003534 EL297_RS11420 WP_134990390.1 1922446..1923672(-) (pilU) [Neisseria meningitidis strain NCTC10025]
MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVNTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=1003534 EL297_RS11420 WP_134990390.1 1922446..1923672(-) (pilU) [Neisseria meningitidis strain NCTC10025]
ATAAATACCGATAACCTGCACGACATCTTGGACGAAATGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCGCACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCGCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAGTCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACCACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCAGGAATTGGGCCTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCAGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCAACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCCGACGTGGTCTGCATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATCGACATCGCCC
TCAACCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAACAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGATCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.321	88.48	0.392
pilU	Pseudomonas stutzeri DSM 10701	44.382	87.255	0.387
pilU	Vibrio cholerae strain A1552	46.131	82.353	0.38