Detailed information NTG1003268

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABG803_RS02045
• Genome accession: NZ_CP156930
• Coordinates: 438141..439289 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 2024CK-00356
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 433141..444289

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABG803_RS02020 (ABG803_02020)	-	434290..434586 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ABG803_RS02025 (ABG803_02025)	-	434583..435176 (-)	594	WP_003084544.1	YggT family protein	-
ABG803_RS02030 (ABG803_02030)	proC	435187..436010 (-)	824	Protein_404	pyrroline-5-carboxylate reductase	-
ABG803_RS02035 (ABG803_02035)	-	436022..436714 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ABG803_RS02040 (ABG803_02040)	pilT	436929..437963 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ABG803_RS02045 (ABG803_02045)	pilU	438141..439289 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
ABG803_RS02050 (ABG803_02050)	czcD	439297..440196 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ABG803_RS02055 (ABG803_02055)	-	440351..440755 (+)	405	WP_003084560.1	NINE protein	-
ABG803_RS02060 (ABG803_02060)	-	440998..442371 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
ABG803_RS02065 (ABG803_02065)	-	442368..443552 (+)	1185	WP_347914800.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1003268 ABG803_RS02045 WP_003100959.1 438141..439289(+) (pilU) [Pseudomonas aeruginosa strain 2024CK-00356]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1003268 ABG803_RS02045 WP_003100959.1 438141..439289(+) (pilU) [Pseudomonas aeruginosa strain 2024CK-00356]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGTA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382