Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AAGD24_RS04785 Genome accession   NZ_CP156894
Coordinates   1059056..1060120 (+) Length   354 a.a.
NCBI ID   WP_036203576.1    Uniprot ID   A0A1W6KDI5
Organism   Marinobacter sp. M5B strain M5B10.3.1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1054056..1065120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGD24_RS04770 rpmE 1054542..1054763 (+) 222 WP_007154619.1 50S ribosomal protein L31 -
  AAGD24_RS04775 - 1054996..1056261 (+) 1266 WP_036203569.1 malic enzyme-like NAD(P)-binding protein -
  AAGD24_RS04780 - 1056355..1058841 (-) 2487 WP_036203572.1 penicillin-binding protein 1A -
  AAGD24_RS04785 pilM 1059056..1060120 (+) 1065 WP_036203576.1 pilus assembly protein PilM Machinery gene
  AAGD24_RS04790 - 1060120..1060683 (+) 564 WP_041335802.1 PilN domain-containing protein -
  AAGD24_RS04795 - 1060683..1061294 (+) 612 WP_379866708.1 type 4a pilus biogenesis protein PilO -
  AAGD24_RS04800 - 1061297..1061845 (+) 549 WP_036203584.1 pilus assembly protein PilP -
  AAGD24_RS04805 pilQ 1061912..1063993 (+) 2082 WP_177190642.1 type IV pilus secretin PilQ Machinery gene
  AAGD24_RS04810 aroK 1064124..1064690 (+) 567 WP_036203590.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38048.10 Da        Isoelectric Point: 4.1243

>NTDB_id=1003070 AAGD24_RS04785 WP_036203576.1 1059056..1060120(+) (pilM) [Marinobacter sp. M5B strain M5B10.3.1]
MFGLLGKKSSSVLGVDISSSSVKLLELSKQGDRYKVESYAVEPLPANAVVEKNITDVEAVGEVLKRVASKSRTSVKQVAV
AVSGSAVITKLIQMDGGLNEFEMEDQITLEADQYIPYPLDEVAIDFEVQGPSEANPDQVDVLLAACRKENVDIREDALEI
ASLTTKIVDVEAYALERAYALIEPQLDSQGEELVVAIVDIGATMTTLSVLAEGKTVYTREQIFGGKQLTEEIQRRYGLSL
EEAGLAKKQGGLPDDYDSEVLTPFREAVVQQVARALQFFFGASQYNAVDYVVLAGGTASIQGLTEMVEEKTGTPTLVANP
FADMAVGSRVNASSLSNDAPSLMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1003070 AAGD24_RS04785 WP_036203576.1 1059056..1060120(+) (pilM) [Marinobacter sp. M5B strain M5B10.3.1]
GTGTTCGGATTGTTAGGAAAAAAATCCAGTTCGGTGCTGGGCGTGGATATCAGTTCCAGCTCGGTCAAGTTACTGGAACT
ATCGAAGCAGGGCGACCGTTACAAGGTCGAGAGCTACGCAGTGGAGCCGCTACCGGCCAATGCTGTGGTAGAGAAAAACA
TCACGGATGTCGAGGCGGTGGGCGAAGTGCTCAAACGTGTCGCGTCCAAATCACGTACGAGCGTCAAGCAGGTGGCTGTG
GCCGTCTCCGGTTCCGCCGTTATTACCAAGCTGATCCAGATGGACGGTGGTCTTAACGAGTTTGAGATGGAAGACCAGAT
CACGCTCGAGGCCGATCAGTATATTCCCTATCCGTTGGATGAAGTGGCAATCGACTTCGAGGTTCAGGGGCCCTCTGAGG
CTAACCCGGATCAGGTCGATGTACTTCTGGCTGCGTGTCGCAAAGAAAACGTCGATATTCGGGAAGACGCTCTGGAGATT
GCTTCCTTGACCACCAAGATTGTGGACGTCGAAGCATACGCACTCGAGCGGGCATACGCCCTCATCGAGCCTCAGCTTGA
TTCCCAGGGTGAAGAACTCGTTGTGGCGATCGTCGATATCGGCGCAACCATGACCACACTGAGCGTGTTGGCTGAAGGCA
AGACGGTTTACACTCGCGAGCAGATTTTCGGGGGGAAACAACTCACCGAGGAAATTCAGCGTCGTTATGGTCTGTCCCTT
GAAGAGGCCGGCCTTGCCAAGAAGCAGGGTGGCCTCCCCGATGACTACGACTCCGAAGTGCTGACGCCTTTCCGGGAAGC
GGTAGTTCAGCAGGTTGCGAGGGCGCTGCAGTTCTTCTTCGGCGCAAGCCAGTACAATGCGGTTGACTACGTAGTTCTGG
CTGGCGGCACGGCATCTATCCAGGGGCTGACAGAAATGGTCGAGGAAAAAACGGGCACACCCACGCTGGTTGCAAATCCG
TTCGCGGACATGGCAGTGGGATCAAGGGTGAATGCGTCTTCGCTGAGCAATGATGCGCCTTCACTCATGATTGCCTGTGG
GCTGGCAATGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W6KDI5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

53.955

100

0.54
  comM Acinetobacter nosocomialis M2

53.672

100

0.537
  comM Acinetobacter baylyi ADP1

51.695

100

0.517
  pilM Legionella pneumophila strain ERS1305867

45.763

100

0.458

Multiple sequence alignment