Detailed information NTG1002891

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABEF84_RS08445
• Genome accession: NZ_CP156795
• Coordinates: 1711494..1712699 (-)
• Length: 401 a.a.
• NCBI ID: WP_034588274.1
• Uniprot ID: A0A0A3X0K5
• Organism: Acinetobacter thermotolerans strain ANC 7912
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1706494..1717699

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABEF84_RS08425 (ABEF84_08425)	-	1707937..1708386 (+)	450	WP_034588266.1	MaoC family dehydratase	-
ABEF84_RS08430 (ABEF84_08430)	-	1708538..1709566 (+)	1029	WP_034588270.1	AraC family transcriptional regulator	-
ABEF84_RS08435 (ABEF84_08435)	gcvH	1709639..1710013 (+)	375	WP_034588271.1	glycine cleavage system protein GcvH	-
ABEF84_RS08440 (ABEF84_08440)	-	1710250..1711443 (+)	1194	WP_034588273.1	MFS transporter	-
ABEF84_RS08445 (ABEF84_08445)	pilU	1711494..1712699 (-)	1206	WP_034588274.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ABEF84_RS08450 (ABEF84_08450)	pilU	1713005..1714183 (-)	1179	WP_034588275.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ABEF84_RS08455 (ABEF84_08455)	-	1714396..1715445 (-)	1050	WP_034588278.1	3-deoxy-7-phosphoheptulonate synthase	-
ABEF84_RS08460 (ABEF84_08460)	-	1716218..1717327 (-)	1110	WP_034588280.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-

Sequence

Protein

• Length: 401 a.a.
• Molecular weight: 45328.66 Da
• Isoelectric Point: 6.2782

>NTDB_id=1002891 ABEF84_RS08445 WP_034588274.1 1711494..1712699(-) (pilU) [Acinetobacter thermotolerans strain ANC 7912]
MFTAELLEEARKFMHHMLTKVVEYGGSDLFITADFPPSIKHQGLMKPFGQQNLTADKTKLFAYALMNEKQRQEYETELEC
NFAITVPGVSRFRVNVFQQQLNVGMVIRTITAEIPNFQKLKLPESLKHVIMEKRGLVLVVGGTGSGKSTSLAAMIDYRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDSHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLIRTQDGQGRRAAVEIMLNTPLISDLILKGDFHELKAIMAKS
RELGMQTFDQALFDLYNEGAISYEEALRNADSTNELRLQIKLKSSRANPELNNNPTSFSMVEEKKPEDEADGESKTEEQT
S

Nucleotide

• Length: 1206 bp

>NTDB_id=1002891 ABEF84_RS08445 WP_034588274.1 1711494..1712699(-) (pilU) [Acinetobacter thermotolerans strain ANC 7912]
ATGTTCACGGCAGAGTTATTAGAAGAAGCACGTAAATTTATGCACCATATGCTCACCAAAGTGGTGGAATATGGTGGTTC
GGATTTATTTATTACTGCCGACTTTCCACCGAGTATCAAACATCAGGGGCTGATGAAACCTTTTGGTCAGCAAAACCTAA
CTGCAGATAAAACCAAGCTGTTTGCTTATGCGTTGATGAATGAAAAACAGCGTCAGGAATACGAAACTGAGCTGGAATGT
AACTTTGCCATTACCGTGCCGGGTGTTTCCCGTTTCCGTGTCAATGTTTTTCAGCAACAGCTCAATGTAGGAATGGTGAT
CCGTACCATTACTGCAGAAATTCCGAACTTCCAGAAACTGAAATTACCTGAATCACTCAAACATGTGATTATGGAAAAAC
GCGGGCTAGTATTGGTTGTTGGGGGGACTGGGTCTGGTAAATCGACCTCACTGGCAGCGATGATTGATTACCGGAATGAA
AATTCCGCCGGACATATCATTACCGTAGAAGATCCGGTGGAATATGTGCATAAGCATAAAAAGTCCATGATCACCCACCG
TGAAGTCGGTGTAGACAGTCACTCCTGGCACAATGCGCTAAAAAATACCCTACGTCAGGCGCCGGATGTGATTCTGATTG
GCGAGATCCGTGATACCGAAACCATGGAACACGCGATTGCCTTTGCCGAAACTGGACACTTATGTCTAGGAACACTACAT
GCCAACAACGCCAACCAGACTTTGGATCGGATTATTAACTTCTTCCCGGAAGAGCGCCGTAATCAGTTGTTGATGGATCT
GTCTTCCAATATGAAAGCCATTATTTCCCAGCGTCTAATTCGTACTCAAGATGGTCAAGGCCGTCGTGCAGCAGTGGAGA
TCATGTTAAATACGCCGCTGATTTCTGACCTGATTTTAAAAGGCGATTTCCATGAGTTGAAAGCAATTATGGCCAAATCC
CGTGAACTCGGTATGCAGACTTTCGATCAGGCACTGTTCGACTTGTACAACGAAGGGGCGATTTCTTATGAAGAGGCACT
ACGTAATGCAGATTCAACTAATGAATTGCGTTTACAAATTAAACTAAAAAGTAGTCGAGCTAATCCTGAGCTGAATAATA
ATCCTACTTCATTCAGTATGGTTGAAGAAAAAAAGCCGGAAGATGAAGCTGATGGCGAAAGCAAGACTGAAGAACAGACT
TCTTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (116-282)

3D structure

• AlphaFold DB: A0A0A3X0K5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60.057	86.783	0.521
pilU	Acinetobacter baylyi ADP1	56.425	89.277	0.504
pilU	Vibrio cholerae strain A1552	52.394	88.529	0.464