Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ABEF85_RS13565 Genome accession   NZ_CP156776
Coordinates   2783160..2783915 (-) Length   251 a.a.
NCBI ID   WP_404798872.1    Uniprot ID   -
Organism   Acinetobacter thermotolerans strain ANC 7955     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2778160..2788915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEF85_RS13545 (ABEF85_13545) aroB 2778770..2779849 (-) 1080 WP_347456725.1 3-dehydroquinate synthase -
  ABEF85_RS13550 (ABEF85_13550) aroK 2779878..2780414 (-) 537 WP_347454501.1 shikimate kinase AroK -
  ABEF85_RS13555 (ABEF85_13555) pilQ 2780451..2782616 (-) 2166 WP_347463951.1 type IV pilus secretin PilQ family protein Machinery gene
  ABEF85_RS13560 (ABEF85_13560) pilP 2782633..2783160 (-) 528 WP_347463953.1 pilus assembly protein PilP Machinery gene
  ABEF85_RS13565 (ABEF85_13565) pilO 2783160..2783915 (-) 756 WP_404798872.1 type 4a pilus biogenesis protein PilO Machinery gene
  ABEF85_RS13570 (ABEF85_13570) pilN 2783912..2784556 (-) 645 WP_347453499.1 PilN domain-containing protein Machinery gene
  ABEF85_RS13575 (ABEF85_13575) pilM 2784556..2785614 (-) 1059 WP_347455476.1 pilus assembly protein PilM Machinery gene
  ABEF85_RS13580 (ABEF85_13580) ponA 2785781..2788312 (+) 2532 WP_347463955.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 251 a.a.        Molecular weight: 28610.49 Da        Isoelectric Point: 5.4805

>NTDB_id=1002646 ABEF85_RS13565 WP_404798872.1 2783160..2783915(-) (pilO) [Acinetobacter thermotolerans strain ANC 7955]
MSEQFEDLGQEPVVAPKNKMTVEKFFQQFNTLDPNNYGSWPIAVKITCWIFIVFVVMMLAYFGLIRGQIEAIAQANAQEQ
NLLNEFRDKDSKLRNLQQYQQQLQEMEARFNQQLEQLPKETEIPGLVEDINLSGVNAGLKFKNIRLEPEVKQEFFIEQPI
SIEATGDYHSFGSFVSGIAGLSRIVTLHDFTITGTENKEKKTDIPVIDYVVKAKTYRYIGTQTGTQTDSDEVGDNDKKKN
KQNKAKKGGKK

Nucleotide


Download         Length: 756 bp        

>NTDB_id=1002646 ABEF85_RS13565 WP_404798872.1 2783160..2783915(-) (pilO) [Acinetobacter thermotolerans strain ANC 7955]
ATGAGCGAACAATTTGAAGATTTAGGTCAAGAGCCAGTTGTTGCACCGAAAAATAAAATGACGGTGGAGAAGTTTTTCCA
GCAGTTTAATACCCTGGATCCAAATAATTATGGTAGCTGGCCGATCGCAGTTAAAATTACCTGCTGGATTTTTATTGTCT
TTGTGGTAATGATGCTGGCTTATTTTGGTTTGATCCGCGGCCAAATCGAAGCCATTGCACAAGCGAATGCTCAGGAGCAA
AACTTGCTCAATGAGTTCCGTGATAAGGATTCCAAGCTGCGTAACTTGCAACAATATCAGCAACAATTGCAGGAAATGGA
AGCACGTTTTAACCAGCAACTCGAGCAGTTGCCGAAAGAAACAGAAATTCCTGGATTGGTTGAAGATATTAACTTGAGCG
GTGTCAACGCCGGCCTGAAGTTCAAAAATATACGTCTTGAACCAGAAGTGAAACAGGAGTTCTTTATCGAACAACCAATT
TCGATTGAGGCAACTGGCGATTATCATTCCTTTGGTTCGTTTGTCAGTGGTATCGCAGGTCTGTCCCGTATAGTCACTTT
ACATGATTTCACGATTACTGGCACTGAAAATAAAGAAAAGAAAACAGATATTCCAGTGATTGATTATGTGGTGAAAGCAA
AGACTTATCGTTATATAGGTACACAGACTGGAACTCAGACTGATTCTGATGAAGTAGGTGATAATGATAAGAAAAAGAAC
AAACAAAATAAGGCTAAAAAAGGAGGGAAGAAGTGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(72-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

69.469

90.04

0.625
  comO Acinetobacter baylyi ADP1

75.61

81.673

0.618

Multiple sequence alignment