Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   EL327_RS11465 Genome accession   NZ_LR134342
Coordinates   2379577..2380200 (-) Length   207 a.a.
NCBI ID   WP_003116135.1    Uniprot ID   A0A0H2ZIF6
Organism   Pseudomonas aeruginosa strain NCTC10728     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2374577..2385200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL327_RS11445 (NCTC10728_02239) aroB 2375184..2376290 (-) 1107 WP_016253983.1 3-dehydroquinate synthase -
  EL327_RS11450 (NCTC10728_02240) aroK 2376340..2376858 (-) 519 WP_003095833.1 shikimate kinase AroK -
  EL327_RS11455 (NCTC10728_02241) pilQ 2376870..2379002 (-) 2133 WP_003116137.1 type 4a pilus secretin PilQ Machinery gene
  EL327_RS11460 (NCTC10728_02242) comL/pilP 2379056..2379580 (-) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  EL327_RS11465 (NCTC10728_02243) pilO 2379577..2380200 (-) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO Machinery gene
  EL327_RS11470 (NCTC10728_02244) pilN 2380197..2380793 (-) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  EL327_RS11475 (NCTC10728_02245) pilM 2380793..2381857 (-) 1065 WP_034024943.1 type IV pilus assembly protein PilM Machinery gene
  EL327_RS11480 (NCTC10728_02246) - 2382042..2384510 (+) 2469 WP_003114576.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22958.35 Da        Isoelectric Point: 4.9107

>NTDB_id=1002193 EL327_RS11465 WP_003116135.1 2379577..2380200(-) (pilO) [Pseudomonas aeruginosa strain NCTC10728]
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLRDLQTQLEQQAAQEESLKQQFATKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPASPDNTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=1002193 EL327_RS11465 WP_003116135.1 2379577..2380200(-) (pilO) [Pseudomonas aeruginosa strain NCTC10728]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTCCGTGACT
TGCAAACACAGCTCGAACAGCAGGCTGCGCAAGAAGAATCGCTCAAGCAGCAATTTGCTACCAAAGCGTTCCAGGCCGCG
AACTTGGAAGCCTACAAGGCACAGATGAAAGAGATGGAGGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGTCTGGGCAGCGGCCTGGAGTTCGAGGAGATCAAGCTGCTCC
CTGAAGTCACTCAGCAGTTCTATATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTTACCCTGCATGACTTCGAGATCAAGCCGGCTTCGCCCGATAACACCTC
GAAGCTGCGCATGAGCATCCTGGCCAAGACCTACCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZIF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

45.596

93.237

0.425
  pilO Acinetobacter baumannii D1279779

45.789

91.787

0.42
  comO/pilO Acinetobacter baylyi ADP1

43.617

90.821

0.396

Multiple sequence alignment