Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   EL144_RS05580 Genome accession   NZ_LR134327
Coordinates   1151213..1152637 (+) Length   474 a.a.
NCBI ID   WP_032995306.1    Uniprot ID   -
Organism   Aggregatibacter aphrophilus ATCC 33389 strain NCTC 5906     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1146213..1157637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL144_RS05555 (NCTC5906_01123) - 1146255..1148816 (-) 2562 WP_050332893.1 penicillin-binding protein 1A -
  EL144_RS05560 (NCTC5906_01124) comA 1148943..1149746 (+) 804 WP_005701812.1 hypothetical protein Machinery gene
  EL144_RS05565 (NCTC5906_01125) - 1149760..1150278 (+) 519 WP_032995314.1 PilN domain-containing protein -
  EL144_RS05570 (NCTC5906_01126) - 1150275..1150808 (+) 534 WP_005704484.1 competence protein -
  EL144_RS05575 (NCTC5906_01127) - 1150808..1151203 (+) 396 WP_032995307.1 hypothetical protein -
  EL144_RS05580 (NCTC5906_01128) comE 1151213..1152637 (+) 1425 WP_032995306.1 type IV pilus secretin PilQ Machinery gene
  EL144_RS05585 (NCTC5906_01129) aroK 1152852..1153379 (+) 528 WP_005704481.1 shikimate kinase AroK -
  EL144_RS05590 (NCTC5906_01130) aroB 1153401..1154489 (+) 1089 WP_005704480.1 3-dehydroquinate synthase -
  EL144_RS05595 (NCTC5906_01131) - 1154495..1155349 (+) 855 WP_050332891.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  EL144_RS05600 (NCTC5906_01132) - 1155655..1156023 (+) 369 WP_005704478.1 rhodanese-like domain-containing protein -
  EL144_RS05605 (NCTC5906_01133) ygiD 1156302..1157087 (-) 786 WP_032995305.1 4,5-DOPA dioxygenase extradiol -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 52278.15 Da        Isoelectric Point: 7.2656

>NTDB_id=1001744 EL144_RS05580 WP_032995306.1 1151213..1152637(+) (comE) [Aggregatibacter aphrophilus ATCC 33389 strain NCTC 5906]
MKLPLPYRVKCGLFFSCFLSFAMSLSVHAEQNQVFSIRLKQAPLVPTLQQLALAQNVNLIIDDELQGTVSLQLENVDLDQ
LFRSVAKIKQLDLWQENGIYYLSKPDTSAKFATKMDEPFILPAVMTEEPTRLTTATIKLHFAKASEVMKSLTGGSGSLLS
PNGSLTFDDRSNLLLIQDEPKSIANLKKLIKELDKPIEQIAIEARIVTMNGESLKELGVRWGIFNPTENAHRIGGSLAGN
GFDNITNQLNVNFPTATTPAGSVALQVAKINGRLLDLELTALERENNVEIIASPRLLTTNKKAASIKQGTELPYILVNEK
SGSQSVEFREAVLGLEVTPHISQDKQILLDLVVSQNAPGSRVAHGLGEVVSIDKQEINTQVFAKDGETIVLGGVFHDTIT
KNVDKVPLLGDIPGVKHLFSKESERHQKRELVIFVTPHILRQGETLESFKQKQGITQQTKMLPTQNTHKNPTKS

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=1001744 EL144_RS05580 WP_032995306.1 1151213..1152637(+) (comE) [Aggregatibacter aphrophilus ATCC 33389 strain NCTC 5906]
ATGAAATTACCTCTGCCCTACCGCGTAAAGTGCGGTCTGTTTTTCAGTTGTTTTTTGAGTTTTGCCATGAGCCTTTCCGT
TCATGCAGAACAAAATCAGGTATTTTCCATTCGGCTGAAACAAGCGCCCTTGGTACCCACTTTGCAACAATTGGCGTTGG
CGCAAAATGTCAATTTGATTATTGATGATGAATTGCAAGGCACGGTTTCGCTCCAACTGGAAAACGTCGATTTGGATCAG
CTGTTTCGCTCCGTTGCTAAAATTAAGCAGTTGGATTTGTGGCAGGAAAATGGGATTTATTATTTAAGCAAACCCGATAC
TTCCGCTAAATTTGCCACCAAAATGGACGAACCCTTTATCCTTCCTGCCGTGATGACGGAAGAACCGACGCGACTCACCA
CCGCCACCATTAAATTGCATTTTGCCAAAGCCTCGGAAGTGATGAAATCGCTCACCGGCGGTAGCGGTTCGTTACTTTCC
CCAAACGGTAGCCTCACTTTTGACGATCGCAGTAACTTGTTATTAATTCAAGACGAACCGAAATCCATCGCTAACCTGAA
AAAACTGATTAAAGAATTAGATAAGCCCATTGAACAAATCGCCATTGAAGCCAGAATTGTCACCATGAACGGCGAAAGTC
TAAAAGAATTAGGCGTACGCTGGGGCATTTTTAACCCCACCGAAAATGCGCATCGTATCGGCGGAAGTTTGGCGGGTAAC
GGCTTTGACAATATCACCAATCAATTAAACGTCAACTTCCCTACAGCCACCACCCCCGCCGGCTCCGTCGCACTACAGGT
GGCGAAAATAAACGGACGACTTTTGGACTTAGAATTGACCGCACTTGAACGGGAAAACAATGTAGAAATTATTGCCAGCC
CGCGATTGCTCACAACCAATAAAAAAGCCGCCAGCATCAAGCAAGGGACGGAATTGCCGTATATTTTGGTGAATGAAAAA
AGCGGCTCACAAAGCGTGGAATTTCGTGAGGCTGTATTAGGCTTGGAAGTAACACCGCATATTTCACAAGATAAACAAAT
TCTGTTGGATTTAGTGGTCAGTCAAAATGCGCCCGGTAGCCGAGTAGCACATGGTTTGGGCGAAGTCGTCTCCATTGATA
AACAGGAAATCAACACCCAAGTCTTTGCTAAAGACGGAGAAACCATCGTGTTAGGTGGCGTATTTCACGATACCATCACT
AAAAACGTGGACAAAGTGCCCTTGTTGGGAGACATTCCGGGCGTAAAACACTTGTTTAGCAAAGAAAGCGAACGTCATCA
AAAACGGGAATTGGTGATTTTTGTCACACCGCATATTTTACGTCAAGGAGAAACCTTAGAATCCTTTAAACAAAAGCAAG
GCATAACACAGCAAACTAAAATGCTCCCCACACAAAACACGCATAAAAATCCAACAAAATCGTAG

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(134-199)

Bacterial type II and III secretion system protein (Secretin)

(281-441)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

69.731

94.093

0.656
  comE Haemophilus influenzae 86-028NP

68.527

94.515

0.648
  comE Glaesserella parasuis strain SC1401

51.818

92.827

0.481
  pilQ Vibrio campbellii strain DS40M4

41.23

92.616

0.382
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.958

90.506

0.38
  pilQ Vibrio cholerae strain A1552

41.958

90.506

0.38