Detailed information NTG1001650

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABEP14_RS02240
• Genome accession: NZ_CP156078
• Coordinates: 487561..488709 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain P2550
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 482561..493709

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABEP14_RS02215 (ABEP14_02215)	-	483712..484008 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ABEP14_RS02220 (ABEP14_02220)	-	484005..484598 (-)	594	WP_003084544.1	YggT family protein	-
ABEP14_RS02225 (ABEP14_02225)	proC	484609..485430 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
ABEP14_RS02230 (ABEP14_02230)	-	485442..486134 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ABEP14_RS02235 (ABEP14_02235)	pilT	486349..487383 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ABEP14_RS02240 (ABEP14_02240)	pilU	487561..488709 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
ABEP14_RS02245 (ABEP14_02245)	czcD	488717..489616 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ABEP14_RS02250 (ABEP14_02250)	-	489771..490175 (+)	405	WP_003084560.1	NINE protein	-
ABEP14_RS02255 (ABEP14_02255)	-	490418..491791 (+)	1374	WP_003459266.1	pyridoxal-phosphate dependent enzyme	-
ABEP14_RS02260 (ABEP14_02260)	-	491788..492972 (+)	1185	WP_016852373.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1001650 ABEP14_RS02240 WP_003100959.1 487561..488709(+) (pilU) [Pseudomonas aeruginosa strain P2550]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1001650 ABEP14_RS02240 WP_003100959.1 487561..488709(+) (pilU) [Pseudomonas aeruginosa strain P2550]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382