Detailed information NTG1001511

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABFK61_RS13680
• Genome accession: NZ_CP156041
• Coordinates: 2861981..2863099 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain D78
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2856981..2868099

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABFK61_RS13650 (ABFK61_13650)	-	2857810..2858658 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
ABFK61_RS13655 (ABFK61_13655)	-	2858911..2859366 (+)	456	WP_000782976.1	bacteriohemerythrin	-
ABFK61_RS13660 (ABFK61_13660)	-	2859519..2860586 (+)	1068	WP_000092403.1	hypothetical protein	-
ABFK61_RS13665 (ABFK61_13665)	-	2860586..2860909 (+)	324	WP_000442585.1	RnfH family protein	-
ABFK61_RS13670 (ABFK61_13670)	bamE	2860964..2861362 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
ABFK61_RS13675 (ABFK61_13675)	fur	2861474..2861911 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
ABFK61_RS13680 (ABFK61_13680)	pilU	2861981..2863099 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ABFK61_RS13685 (ABFK61_13685)	pilT	2863127..2864164 (-)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
ABFK61_RS13690 (ABFK61_13690)	-	2864291..2864983 (+)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=1001511 ABFK61_RS13680 WP_000347036.1 2861981..2863099(-) (pilU) [Acinetobacter baumannii strain D78]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=1001511 ABFK61_RS13680 WP_000347036.1 2861981..2863099(-) (pilU) [Acinetobacter baumannii strain D78]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392