Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABFU68_RS05295 Genome accession   NZ_CP155997
Coordinates   1243444..1245177 (+) Length   577 a.a.
NCBI ID   WP_012437606.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain LMG 00583     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1238444..1250177
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU68_RS05275 (ABFU68_05275) comP 1239130..1239543 (+) 414 WP_012437602.1 pilin Machinery gene
  ABFU68_RS05280 (ABFU68_05280) - 1239762..1241273 (+) 1512 WP_256729490.1 phosphoethanolamine transferase -
  ABFU68_RS05285 (ABFU68_05285) - 1241284..1241913 (+) 630 WP_012437604.1 hypothetical protein -
  ABFU68_RS05290 (ABFU68_05290) - 1241883..1243409 (+) 1527 WP_016944997.1 membrane protein -
  ABFU68_RS05295 (ABFU68_05295) pilB 1243444..1245177 (+) 1734 WP_012437606.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFU68_RS05300 (ABFU68_05300) pilR 1245594..1246988 (-) 1395 WP_012437607.1 sigma-54 dependent transcriptional regulator Regulator
  ABFU68_RS05305 (ABFU68_05305) - 1247197..1248807 (-) 1611 WP_012437608.1 PAS domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62661.87 Da        Isoelectric Point: 5.7669

>NTDB_id=1000936 ABFU68_RS05295 WP_012437606.1 1243444..1245177(+) (pilB) [Xanthomonas campestris pv. campestris strain LMG 00583]
MNSVATTNLVGITGIARRLVQDGALEEAAARTAMEQAAAAKVPLPQWFAEKKLVSAAQLAAANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTKALDDIKFHTNLVVEPILVDEDQIRRTLEQWQAGNASFGSALG
GDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1000936 ABFU68_RS05295 WP_012437606.1 1243444..1245177(+) (pilB) [Xanthomonas campestris pv. campestris strain LMG 00583]
ATGAATTCAGTAGCTACCACTAACCTCGTTGGCATCACCGGCATCGCTCGTCGCCTCGTGCAGGATGGCGCCCTGGAGGA
AGCCGCTGCGCGGACGGCGATGGAACAGGCGGCCGCTGCCAAGGTGCCGCTGCCGCAGTGGTTTGCCGAGAAGAAGCTGG
TGTCGGCGGCGCAGCTGGCGGCGGCCAATGCCGTGGAATTCGGCATGCCGCTAATGGATGTGTCGGTGTTTGACGCTAGT
CAGAACGCGGTGAAGCTGGTAAGTGAAGAGTTGCTCCAGAAGCATCAGGTGTTGCCGCTGTTCAAGCGCGGCAACCGATT
GTTTGTGGGGGTGAGCAACCCGACGCAGACCAAGGCATTGGACGACATCAAGTTCCATACCAACTTGGTGGTGGAGCCAA
TTCTGGTCGATGAGGACCAGATCCGTAGGACGTTGGAGCAATGGCAGGCAGGAAACGCGTCCTTTGGCTCGGCGCTTGGT
GGCGACGACGAGGAGATGGGGGACCTAGACGTGTCCGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGTGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGTCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGATTTTCGCGTGAGCACCTTGCCGACCTTGTTCGGCGAAA
AGGTGGTGCTGCGTATCCTGGACGCCAGTGCGGCCAAGCTCGGTATCGAGAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTATACCGCGCTCGGGATTCTCAACGACGAGACGCGCAACATTTCCACGGCCGAGGATCCGGTCGAAATCCGCCTGCCTG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACGTTTGCCGCTGCGCTGCGCTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCGACGCTGCACACCAACGATGCGCCGCAAACCATTGCGCGTCTGATGAACATGGGTATCGCACCGTACAACATCA
CCTCCTCGGTGACCTTGGTCATCGCGCAGCGCCTGGCGCGGCGGCTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTTGCGAACATCGAGCTCTATGAAGCGGTCGGTTGCGATGA
GTGCACGGAAGGCTACAAGGGCCGTACCGGCATCTATCAGGTAATGCCGATGACCGATGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCACAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTGATGAAG
GCTTCGCACGGCGTCACCAGCCTCGCCGAGATCAATCGTGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.514

98.44

0.556
  pilB Acinetobacter baylyi ADP1

55.652

99.653

0.555
  pilB Legionella pneumophila strain ERS1305867

52.465

98.44

0.516
  pilB Vibrio cholerae strain A1552

49.567

100

0.496
  pilF Neisseria gonorrhoeae MS11

49.12

98.44

0.484
  pilB Vibrio campbellii strain DS40M4

46.046

98.614

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

46.333

96.88

0.449

Multiple sequence alignment