Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   ABFU65_RS04565 Genome accession   NZ_CP155964
Coordinates   1076973..1078031 (+) Length   352 a.a.
NCBI ID   WP_016944654.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain BR25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1071973..1083031
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU65_RS04550 (ABFU65_04550) - 1073102..1073752 (-) 651 WP_042597625.1 hypothetical protein -
  ABFU65_RS04560 (ABFU65_04560) - 1074331..1076757 (-) 2427 WP_323549531.1 penicillin-binding protein 1A -
  ABFU65_RS04565 (ABFU65_04565) comM 1076973..1078031 (+) 1059 WP_016944654.1 pilus assembly protein PilM Machinery gene
  ABFU65_RS04570 (ABFU65_04570) - 1078031..1078810 (+) 780 WP_228442753.1 PilN domain-containing protein -
  ABFU65_RS04575 (ABFU65_04575) - 1078807..1079472 (+) 666 WP_011038333.1 type 4a pilus biogenesis protein PilO -
  ABFU65_RS04580 (ABFU65_04580) - 1079469..1080002 (+) 534 WP_011038332.1 pilus assembly protein PilP -
  ABFU65_RS04585 (ABFU65_04585) - 1080022..1081965 (+) 1944 WP_014508815.1 type IV pilus secretin PilQ -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37620.24 Da        Isoelectric Point: 4.3924

>NTDB_id=1000368 ABFU65_RS04565 WP_016944654.1 1076973..1078031(+) (comM) [Xanthomonas campestris pv. raphani strain BR25]
MGLLPKSQSPLIGVDISSTAVKLLQLSRSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAINRSGSKAKNAAAA
VAGSAVITKLIPMPADLDDSDLEAQVELEATNYIPYPIEEVNLDFEVLGPMPNSPDMVQVLLAASRSENVELRQSALELG
GLVAKVMDVEAFAVENAFALVASELPVAADAVVALVDIGATMTTLSVLRSGRSLYSREQVFGGKQLTDEVMRRYGLTYEE
AGLAKRQGGLPESYEVEVLEPFKEATVQQISRLLQFFYAGSEFNRVDCIVLAGGCAALSRLPEMVEEQLGVTTVVANPLA
QMTLGPKVQAHALALDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=1000368 ABFU65_RS04565 WP_016944654.1 1076973..1078031(+) (comM) [Xanthomonas campestris pv. raphani strain BR25]
GTGGGGCTTTTACCCAAGAGTCAGTCGCCACTTATTGGTGTCGACATCAGTTCCACTGCGGTCAAGCTATTGCAGCTGTC
ACGCAGCGGAAATCGTTTTCGCGTGGAACATTACGCTGTGGAACCGTTGCCGCCGAATGCGGTGGTGGAAAAGAACATCG
TCGAAGTCGAAGCGGTGGGCGAAGCCATTCGCCGCGCCATCAACCGTTCCGGCAGCAAGGCCAAGAACGCGGCAGCGGCC
GTGGCTGGCTCGGCGGTGATCACCAAGCTGATCCCCATGCCGGCGGATCTGGACGATAGCGATCTGGAAGCCCAGGTCGA
GCTGGAAGCCACCAACTACATCCCGTACCCGATCGAGGAAGTGAATCTCGATTTCGAGGTGCTCGGCCCGATGCCCAACA
GCCCGGACATGGTCCAGGTGCTGTTGGCGGCGTCGCGTTCGGAGAACGTCGAGCTGCGCCAATCGGCGTTGGAGCTCGGT
GGGCTGGTCGCCAAGGTGATGGACGTGGAGGCCTTCGCGGTCGAGAACGCCTTTGCCCTGGTTGCCAGCGAATTGCCGGT
CGCCGCCGATGCGGTGGTGGCGCTGGTGGACATCGGCGCGACCATGACCACGTTGAGCGTGCTGCGCTCAGGGCGCAGCC
TCTACAGCCGCGAGCAGGTGTTCGGTGGCAAGCAGCTCACCGACGAAGTCATGCGCCGCTACGGCCTGACCTATGAAGAA
GCTGGCCTGGCCAAGCGTCAGGGCGGCCTGCCGGAGAGCTACGAGGTCGAAGTGCTGGAGCCGTTCAAGGAAGCGACGGT
GCAGCAGATCAGCCGCCTGTTGCAGTTCTTCTATGCGGGCAGCGAATTCAATCGCGTCGATTGCATCGTGCTGGCAGGCG
GTTGCGCGGCGTTGTCGCGCCTGCCCGAGATGGTGGAAGAACAGCTGGGCGTGACTACCGTGGTCGCCAACCCGCTGGCA
CAGATGACGCTGGGCCCGAAGGTTCAGGCCCATGCGCTGGCGCTGGATGCGCCTGCATTGATGATCGCCACCGGCCTGGC
CCTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

51.136

100

0.511
  comM Acinetobacter baylyi ADP1

50.997

99.716

0.509
  pilM Acinetobacter baumannii D1279779

50.852

100

0.509
  pilM Legionella pneumophila strain ERS1305867

47.293

99.716

0.472

Multiple sequence alignment