Detailed information NTG1000336

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: ABFU72_RS05530
• Genome accession: NZ_CP155962
• Coordinates: 1305497..1307203 (+)
• Length: 568 a.a.
• NCBI ID: WP_228943908.1
• Uniprot ID: -
• Organism: Xanthomonas campestris pv. raphani strain NBC269
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	1307313..1308415	1305497..1307203	flank	110

Gene organization within MGE regions

Location: 1305497..1308415

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABFU72_RS05530 (ABFU72_05510)	pilB	1305497..1307203 (+)	1707	WP_228943908.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 568 a.a.
• Molecular weight: 61777.84 Da
• Isoelectric Point: 5.7640

>NTDB_id=1000336 ABFU72_RS05530 WP_228943908.1 1305497..1307203(+) (pilB) [Xanthomonas campestris pv. raphani strain NBC269]
MGITGIARRLVQDGSLEEGAARSAMDQAAAAKIPLPQWFAEKKLVSAAQLAAANAVEFGMPLMDVSVFDASQNAVKLVSE
ELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSMGDDDEGMGDL
DVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLNQRIAA
RLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQKLFLDAIHKP
YGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPEHALLAEGF
TPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMKASHGVTSLA
EINRVTKD

Nucleotide

• Length: 1707 bp

>NTDB_id=1000336 ABFU72_RS05530 WP_228943908.1 1305497..1307203(+) (pilB) [Xanthomonas campestris pv. raphani strain NBC269]
ATCGGAATAACTGGAATAGCCCGTCGTTTGGTACAGGACGGCTCATTGGAAGAAGGCGCTGCGCGTTCAGCAATGGACCA
GGCTGCTGCCGCAAAAATTCCACTGCCTCAGTGGTTTGCTGAAAAAAAATTAGTATCTGCAGCTCAGCTCGCCGCAGCTA
ATGCCGTAGAGTTCGGTATGCCGCTGATGGATGTGTCGGTGTTTGACGCCAGCCAGAACGCGGTCAAGTTGGTCAGTGAG
GAGTTGCTCCAGAAGCATCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGTTTGTTTGTGGGGGTGAGCAACCCGACCCA
GACCCGTGCGCTGGACGACATCAAGTTCCATACTAATCTTGTGGTCGAGCCGATCCTCGTCGACGAGGATCAGATCCGGC
GCACCTTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTCGGTTCGTCGATGGGGGACGACGACGAGGGGATGGGAGACCTA
GACGTGTCCGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCGAAGGGCGATGACACGCCGGTGGT
GAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACATCCATTTCGAGCCGTATGAAGATGACT
ACCGGGTGCGGTTGCGGATTGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCGGTGAAGCTCAACCAGCGCATCGCGGCG
CGCTTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAAGACGGGCGCATCAAGCTCAACCTGTCCAA
GACCAAGCAGATCGATTTTCGCGTGAGCACCTTGCCGACCTTGTTCGGCGAGAAGGTGGTGCTGCGTATCCTGGACGCCA
GCGCGGCCAAGCTCGGTATCGAGAAGCTGGGTTACGAGGCGGATCAGCAGAAGCTGTTCCTGGATGCGATCCACAAGCCC
TACGGCATGGTGCTGGTGACCGGGCCGACTGGTTCGGGCAAGACCGTGTCGCTGTATACCGCGCTCGGGATTCTCAACGA
CGAGACCCGCAATATTTCCACGGCCGAGGATCCGGTCGAAATCCGCCTGCCCGGCGTCAATCAGGTGCAGCAGAACGTCA
AGCGGGGCATGACGTTTGCGGCGGCGCTGCGCTCGTTCCTGCGTCAGGACCCGGACATCATCATGGTCGGCGAAATCCGC
GACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGCCACATGGTGCTGTCGACACTGCACACCAACGATGC
GCCGCAAACCATTGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATCACCTCGTCGGTGACCCTGGTCATCGCGC
AGCGTCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCGGAACATGCGCTGCTGGCGGAAGGCTTT
ACGCCGGAGCAGGTTGCGAACATCGAGCTGTATGAAGCGGTCGGTTGCGATGAATGCACGGAAGGCTACAAGGGCCGTAC
CGGCATCTATCAGGTGATGCCGATGACCGATGAAATCGGCGCGATCGTGCTGGAAGGCGGCAATGCGATGCAGATCGCCG
AGGCTGCGCAGAAGATCGGTATCCGCGACCTGCGTCAGTCGGCATTGATGAAGGCTTCGCACGGCGTGACTAGCCTGGCC
GAGATCAATCGTGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (40-144)

• Type II/IV secretion system protein (T2SSE) (192-459)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	56.162	100	0.562
pilB	Acinetobacter baylyi ADP1	55.458	100	0.555
pilB	Legionella pneumophila strain ERS1305867	52.641	100	0.526
pilF	Neisseria gonorrhoeae MS11	48.592	100	0.486
pilB	Vibrio cholerae strain A1552	49.548	97.359	0.482
pilB	Vibrio campbellii strain DS40M4	46.397	100	0.465
pilB	Vibrio parahaemolyticus RIMD 2210633	46.691	98.415	0.46