Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABFU31_RS03765 Genome accession   NZ_CP155950
Coordinates   854287..855420 (-) Length   377 a.a.
NCBI ID   WP_012439300.1    Uniprot ID   Q93S76
Organism   Xanthomonas campestris pv. raphani strain bglFP 6814     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 849287..860420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU31_RS03735 (ABFU31_03730) - 849310..849645 (-) 336 WP_011035833.1 phasin family protein -
  ABFU31_RS03740 (ABFU31_03735) queD 849835..850188 (-) 354 WP_008576903.1 6-carboxytetrahydropterin synthase QueD -
  ABFU31_RS03745 (ABFU31_03740) - 850304..851692 (-) 1389 WP_011035834.1 DEAD/DEAH box helicase -
  ABFU31_RS03750 (ABFU31_03745) - 852072..853061 (+) 990 WP_050911715.1 fumarylacetoacetate hydrolase family protein -
  ABFU31_RS03755 (ABFU31_03750) maiA 853105..853767 (+) 663 WP_050911714.1 maleylacetoacetate isomerase -
  ABFU31_RS03760 (ABFU31_03755) - 853935..854179 (+) 245 Protein_723 hypothetical protein -
  ABFU31_RS03765 (ABFU31_03760) pilU 854287..855420 (-) 1134 WP_012439300.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFU31_RS03770 (ABFU31_03765) - 855519..855902 (+) 384 WP_011035838.1 DUF4398 domain-containing protein -
  ABFU31_RS03775 (ABFU31_03770) - 855899..856789 (+) 891 WP_407468222.1 hypothetical protein -
  ABFU31_RS03780 (ABFU31_03775) - 857131..857349 (+) 219 WP_019237985.1 YdcH family protein -
  ABFU31_RS03785 (ABFU31_03780) - 857581..858951 (+) 1371 WP_274298692.1 pyridoxal-phosphate dependent enzyme -
  ABFU31_RS03790 (ABFU31_03785) - 859045..860244 (+) 1200 WP_116921851.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41799.10 Da        Isoelectric Point: 6.1401

>NTDB_id=1000126 ABFU31_RS03765 WP_012439300.1 854287..855420(-) (pilU) [Xanthomonas campestris pv. raphani strain bglFP 6814]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALSDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGLDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKTGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYESASAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1000126 ABFU31_RS03765 WP_012439300.1 854287..855420(-) (pilU) [Xanthomonas campestris pv. raphani strain bglFP 6814]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTTTACCCGCTCGGCAATACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCAT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCTCTGTCCGACGCCGGGCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGTGCCATCCGCAGCCGGATCCCGAGCATCGA
GGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGTTCGACCGGCT
CGGGCAAGTCGACCTCGCTGGCCTCAATGATCGACCACCGCAACAGCACCACGACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTAGGCCTGGACACGCATGCTTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCCGACGTGATCCTGATCGGCGAGATCCTGGACGCCGAAACCATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGTCACCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGTGCGCATAAGAACGTGCTGATGAACCTGGCGCTGAATCTGCGCGCAGTGGTGTCCCAGCG
CCTGGTCAAGGGCCTGGACGGGCGCCGGCGCCCGGCCACCGAGGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTAC
GTCGCGGGCAGGTGCACGAGGTCAAGCAGGCGATGGAGGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAGTGCCTG
TTCCGGATGGTCAAGACCGGCGAGATCGAGCAGGAGGAAGCGCTGCGCGCTGCCGACTCGCGCGACGGCCTGGCGCTGAA
ATTCCGTCTTTCCGAAGGCGGCTCCGGGGAGCACGATCCGTATGCCGACTACGAGTCGGCCTCGGCGCCGAAGATCAGCC
ATGGGTTTATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q93S76

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.368

95.225

0.499
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.764

100

0.454
  pilT Pseudomonas aeruginosa PAK

42.604

89.655

0.382
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Acinetobacter baumannii D1279779

41.124

89.655

0.369
  pilT Acinetobacter nosocomialis M2

41.124

89.655

0.369
  pilT Acinetobacter baumannii strain A118

41.124

89.655

0.369
  pilT Acinetobacter baylyi ADP1

40.588

90.186

0.366
  pilT Pseudomonas stutzeri DSM 10701

41.018

88.594

0.363

Multiple sequence alignment