Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   EL191_RS02975 Genome accession   NZ_LR134290
Coordinates   640535..641158 (+) Length   207 a.a.
NCBI ID   WP_017363398.1    Uniprot ID   A0ABN4IYU5
Organism   Ectopseudomonas mendocina strain NCTC10897     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 635535..646158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL191_RS02960 (NCTC10897_00598) - 636307..638718 (-) 2412 WP_041976365.1 penicillin-binding protein 1A -
  EL191_RS02965 (NCTC10897_00599) pilM 638900..639964 (+) 1065 WP_013713726.1 pilus assembly protein PilM Machinery gene
  EL191_RS02970 (NCTC10897_00600) pilN 639964..640548 (+) 585 WP_017363399.1 PilN domain-containing protein Machinery gene
  EL191_RS02975 (NCTC10897_00601) pilO 640535..641158 (+) 624 WP_017363398.1 type 4a pilus biogenesis protein PilO Machinery gene
  EL191_RS02980 comL/pilP 641155..641682 (+) 528 WP_017363397.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  EL191_RS02985 (NCTC10897_00602) pilQ 641737..643817 (+) 2081 Protein_599 type IV pilus secretin PilQ -
  EL191_RS02990 (NCTC10897_00603) aroK 643823..644341 (+) 519 WP_026042192.1 shikimate kinase AroK -
  EL191_RS02995 (NCTC10897_00604) aroB 644393..645496 (+) 1104 WP_017363395.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22970.19 Da        Isoelectric Point: 4.3225

>NTDB_id=1000095 EL191_RS02975 WP_017363398.1 640535..641158(+) (pilO) [Ectopseudomonas mendocina strain NCTC10897]
MSLAESIESLRNFDLNDLDLNNIGSWPAAVKVIICSLFFLLVVGGGYYFHLSDLQNSLDATKAQEESLKQQFSTKAFQAA
NLEAYKEQMAEMELSFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVVQQFYIELPIQIAVEGSYHDLATF
VSGVASLPRIVTLHDFEIKPAAQEGSSVLRMTVLAKTYRYNDKGVEK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=1000095 EL191_RS02975 WP_017363398.1 640535..641158(+) (pilO) [Ectopseudomonas mendocina strain NCTC10897]
ATGAGCCTCGCTGAATCTATCGAGAGTTTGCGTAACTTTGATCTGAATGATCTGGATCTAAATAATATAGGCAGCTGGCC
TGCGGCTGTGAAAGTGATCATTTGTTCGTTGTTTTTCTTGTTGGTTGTTGGTGGTGGGTATTACTTTCATCTTAGCGATT
TACAAAATTCTTTAGATGCTACTAAGGCTCAGGAGGAATCGCTTAAGCAGCAGTTTTCAACTAAAGCCTTTCAAGCTGCA
AATCTCGAGGCTTATAAAGAGCAGATGGCGGAAATGGAGTTGTCTTTTGGTGCTTTGCTTCGGCAGTTGCCAAGTGACAC
TGAAGTGCCTGGGCTTTTGGAGGATATTACTCGTACCGGTCTGGGTAGTGGCTTGGAGTTTGAGGAGATTAAGCTGCTGC
CTGAGGTTGTGCAGCAATTCTACATAGAGTTGCCGATACAAATTGCAGTGGAGGGTAGCTATCATGATCTTGCTACATTC
GTTAGTGGTGTGGCTAGCCTTCCTCGAATAGTAACGTTGCACGACTTTGAAATAAAGCCTGCAGCTCAAGAAGGGTCATC
TGTTTTGCGCATGACTGTTCTGGCGAAGACTTATCGTTATAACGATAAGGGGGTGGAAAAGTGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

45.312

92.754

0.42
  comO/pilO Acinetobacter baylyi ADP1

44.444

91.304

0.406
  pilO Acinetobacter baumannii D1279779

43.684

91.787

0.401