Detailed information NTG1000031

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: EL070_RS03695
• Genome accession: NZ_LR134284
• Coordinates: 702735..704978 (+)
• Length: 747 a.a.
• NCBI ID: WP_111705679.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain NCTC8232
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 697735..709978

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL070_RS03670 (NCTC8232_00724)	-	697966..699966 (+)	2001	WP_111705680.1	potassium transporter Kup	-
EL070_RS03675 (NCTC8232_00725)	-	699990..700268 (-)	279	WP_003061674.1	GIY-YIG nuclease family protein	-
EL070_RS03680 (NCTC8232_00726)	-	700258..701034 (-)	777	WP_111705883.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
EL070_RS03685 (NCTC8232_00727)	-	701152..701892 (+)	741	WP_002989170.1	lysophospholipid acyltransferase family protein	-
EL070_RS03690 (NCTC8232_00728)	-	702092..702754 (+)	663	WP_002983967.1	ComEA family DNA-binding protein	-
EL070_RS03695 (NCTC8232_00729)	comEC/celB	702735..704978 (+)	2244	WP_111705679.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EL070_RS03700 (NCTC8232_00730)	holA	705049..706089 (+)	1041	WP_011017949.1	DNA polymerase III subunit delta	-
EL070_RS03705 (NCTC8232_00731)	sodA	706186..706791 (+)	606	WP_011017948.1	superoxide dismutase SodA	-
EL070_RS03710 (NCTC8232_00732)	-	706958..707104 (+)	147	WP_011017947.1	hypothetical protein	-
EL070_RS03715 (NCTC8232_00733)	-	707160..707408 (+)	249	WP_030127124.1	serine hydrolase	-
EL070_RS03720 (NCTC8232_00734)	-	707405..707995 (+)	591	WP_111705678.1	serine hydrolase	-
EL070_RS03725 (NCTC8232_00735)	-	708109..709329 (-)	1221	WP_111705677.1	DUF3114 domain-containing protein	-

Sequence

Protein

• Length: 747 a.a.
• Molecular weight: 85504.39 Da
• Isoelectric Point: 9.7535

>NTDB_id=1000031 EL070_RS03695 WP_111705679.1 702735..704978(+) (comEC/celB) [Streptococcus pyogenes strain NCTC8232]
MTFSWTKLVPLSKIQFAFLILVLFYQIHSPSWLTFLLSLSLICLLVKRLSKKEFLGVFAILSFCTLFLLYQKQQLVQKLE
IQPVQITSVALVPDSIRINGDQLAVLGRHGKHSYQLFYRLKSQAEAQSFKKEHRWLVMHAKVTLEKAEEVRNFKGFNYQT
FLAYQGIYRIGKVEQIEQLEVISPKSICDYLSSLRRRAIVHCQQHFPRPMSHYLTGLLFGYLDKSFGEMTDYYSQLGIIH
LFALSGMQVGFFLTCFRRVLLLLAVPLEWIKWIELPFACFYAALTGYSISVIRSLVQSQLRHLGIKGLDNLACTFLLVFL
WDAHFLMTVGGVLTFSYAFLLTVVTVEELSGAKRQLVQVLTISLGILPFLLFYFSSFNPMSMVLTALLSYLFDLFILPLL
CLVFCLSPLVTVSICNHLFILLEKVIQFLGNTFNSSLVFGSPTSWHLLILVISFAIFYDYRQVRQRVITCGLVIALTLLS
VKYPLTNEVTFIDIGQGDSILVREWTGKNLLIDVGGRPFFSSKEHWRRGHHVANAQKTLIPYLKSRGIHTIDQLLVTHAD
TDHMGDIEVVAKAIRIKEILTSQGSLSHPSFVRRLRRLKCHVRVLAAGDQLPIMGSVLQVLYPWQLGDGKNNDSLVLYGR
LLNRTFLFTGDLEKEGENEIIKRYPQLRVDYLKAGHHGSNTSSSAAFLDHIQPKVAFISAGKNNRYQHPHRETLARLEDR
QITYYRTDTQGAIRLTGWTSWHLETVR

Nucleotide

• Length: 2244 bp

>NTDB_id=1000031 EL070_RS03695 WP_111705679.1 702735..704978(+) (comEC/celB) [Streptococcus pyogenes strain NCTC8232]
ATGACATTTTCTTGGACTAAGCTAGTGCCCCTGTCAAAGATTCAATTTGCTTTTTTAATCTTGGTTCTCTTTTATCAAAT
CCATAGCCCTAGCTGGTTAACGTTTCTCCTTAGCCTTAGTTTGATTTGCTTACTTGTCAAGCGTTTGTCTAAAAAAGAAT
TTCTCGGTGTCTTTGCCATCCTTAGTTTCTGTACTTTGTTTTTGCTGTATCAGAAACAGCAGTTGGTTCAAAAACTGGAA
ATTCAGCCAGTACAAATAACCTCTGTAGCATTAGTTCCTGATAGTATTCGGATTAATGGTGATCAATTAGCTGTTCTTGG
ACGTCACGGGAAACATTCTTATCAGTTATTTTACCGTTTGAAAAGCCAAGCAGAAGCCCAATCGTTCAAGAAAGAGCACC
GTTGGTTAGTGATGCACGCAAAAGTGACATTGGAAAAAGCTGAGGAAGTTCGAAATTTTAAAGGCTTTAATTATCAGACT
TTTTTGGCTTATCAAGGAATTTACAGGATAGGGAAGGTAGAGCAAATAGAACAGCTTGAAGTGATTTCACCCAAGTCAAT
ATGTGACTATTTGTCCTCTTTAAGACGACGAGCCATTGTTCATTGCCAGCAGCATTTTCCAAGACCTATGTCTCACTACC
TGACGGGCCTCCTTTTTGGTTATTTAGATAAGTCGTTTGGTGAGATGACAGATTATTATAGTCAGTTGGGTATCATTCAT
CTCTTTGCTCTATCAGGGATGCAGGTTGGCTTCTTTCTTACTTGTTTTAGAAGGGTGTTGCTATTACTCGCAGTTCCTTT
GGAATGGATAAAATGGATAGAGCTTCCTTTTGCTTGCTTTTATGCCGCGTTGACAGGTTATAGTATTTCTGTTATTCGAA
GCCTCGTGCAGTCGCAGTTGCGTCATTTAGGAATAAAAGGATTAGACAATCTGGCTTGTACTTTTTTACTTGTCTTTTTA
TGGGATGCCCATTTCTTAATGACTGTTGGTGGCGTATTAACGTTTAGCTATGCTTTTTTGTTAACAGTGGTTACTGTTGA
AGAATTATCAGGAGCTAAAAGACAGTTGGTACAAGTCTTGACCATTTCCCTAGGAATATTACCATTTTTGCTCTTTTATT
TTTCCAGTTTTAACCCAATGTCAATGGTCTTAACAGCTCTCTTGTCTTATCTCTTTGACCTTTTTATTTTGCCCCTTCTT
TGTCTGGTTTTTTGTCTATCCCCTTTGGTGACTGTCTCAATATGCAACCATCTTTTTATTTTATTAGAAAAAGTCATTCA
ATTTCTCGGAAATACTTTCAATAGTTCCCTTGTTTTTGGAAGCCCGACCAGTTGGCACTTGCTTATCTTGGTGATTAGCT
TCGCTATTTTTTATGATTATCGGCAGGTGCGACAACGAGTTATCACTTGCGGTTTGGTTATAGCGTTGACGCTTTTGTCT
GTCAAATATCCTTTGACCAATGAAGTGACCTTTATCGATATTGGCCAGGGAGATAGTATTTTGGTGAGGGAGTGGACAGG
GAAAAATCTCTTAATAGATGTAGGTGGGCGCCCTTTCTTTTCTTCAAAAGAGCACTGGCGACGAGGACATCACGTAGCTA
ATGCTCAGAAAACCTTAATTCCTTACTTAAAAAGTAGAGGTATTCACACAATTGACCAACTATTAGTAACTCACGCAGAT
ACTGATCATATGGGGGATATCGAAGTGGTTGCCAAAGCTATTCGGATAAAGGAGATTTTGACTAGTCAAGGCAGTTTAAG
CCATCCAAGCTTTGTTAGGCGGCTAAGGCGTTTAAAATGTCACGTTAGGGTTTTAGCAGCTGGAGACCAGTTGCCTATTA
TGGGAAGTGTCTTACAGGTACTCTACCCTTGGCAGTTAGGGGACGGGAAAAATAACGATTCTTTGGTACTTTATGGCAGG
CTCTTAAATCGAACTTTTCTTTTCACAGGTGACTTGGAAAAAGAGGGGGAAAACGAGATCATAAAACGTTATCCTCAACT
AAGAGTAGATTATTTAAAAGCAGGTCACCATGGATCAAATACCTCTTCTAGTGCAGCTTTTTTAGACCATATTCAGCCTA
AAGTGGCCTTTATTTCTGCTGGAAAAAATAACCGTTATCAGCATCCTCACAGAGAAACCTTAGCCCGTTTGGAAGACAGA
CAAATTACTTATTACCGAACGGATACGCAAGGAGCTATTCGTTTGACTGGTTGGACAAGCTGGCATCTTGAAACGGTCCG
TTAG

Domains

Predicted by InterProScan.

• Competence protein (Competence) (217-462)

• Metallo-beta-lactamase superfamily (Lactamase_B) (495-701)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus mitis SK321	46.319	100	0.463
comEC/celB	Streptococcus mitis NCTC 12261	46.113	99.866	0.461
comEC/celB	Streptococcus pneumoniae TIGR4	45.114	100	0.451
comEC/celB	Streptococcus pneumoniae Rx1	44.92	100	0.45
comEC/celB	Streptococcus pneumoniae D39	44.92	100	0.45
comEC/celB	Streptococcus pneumoniae R6	44.92	100	0.45
comEC	Lactococcus lactis subsp. cremoris KW2	40.671	99.732	0.406