Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/RHH-PIN |
| Location | 840947..841656 | Replicon | chromosome |
| Accession | NC_000962 | ||
| Organism | Mycobacterium tuberculosis H37Rv | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | P9WF75 |
| Locus tag | Rv0749 | Protein ID | NP_215263.1 |
| Coordinates | 841228..841656 (+) | Length | 143 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | O53811 |
| Locus tag | Rv0748 | Protein ID | NP_215262.1 |
| Coordinates | 840947..841204 (+) | Length | 86 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Rv0747 | 838451..840856 | + | 2406 | YP_177751.1 | PE-PGRS family protein PE_PGRS10 | - |
| Rv0748 | 840947..841204 | + | 258 | NP_215262.1 | antitoxin VapB31 | Antitoxin |
| Rv0749 | 841228..841656 | + | 429 | NP_215263.1 | ribonuclease VapC31 | Toxin |
| Rv0749A | 841737..841874 | - | 138 | YP_177632.1 | hypothetical protein | - |
| Rv0750 | 842033..842278 | + | 246 | NP_215264.1 | hypothetical protein | - |
| Rv0751c | 842347..843231 | - | 885 | NP_215265.1 | 3-hydroxyisobutyrate dehydrogenase | - |
| Rv0752c | 843242..844414 | - | 1173 | NP_215266.1 | acyl-CoA dehydrogenase FadE9 | - |
| Rv0753c | 844421..845953 | - | 1533 | NP_215267.1 | methylmalonate-semialdehyde dehydrogenase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 143 a.a. Molecular weight: 15831.27 Da Isoelectric Point: 7.5536
>T87 NP_215263.1 NC_000962:841228-841656 [Mycobacterium tuberculosis H37Rv]
MFLLDANVLLAAHRGDHPNHRTVRPWFDRLLAADDPFTVPNLVWASFLRLATNRRIFEIPSPRAEAFAFVEAVTAQPHHL
PTNPGPRHLMLLRKLCDEADASGDLIPDAVLAAIAVGHHCAVVSLDRDFARFASVRHIRPPL
MFLLDANVLLAAHRGDHPNHRTVRPWFDRLLAADDPFTVPNLVWASFLRLATNRRIFEIPSPRAEAFAFVEAVTAQPHHL
PTNPGPRHLMLLRKLCDEADASGDLIPDAVLAAIAVGHHCAVVSLDRDFARFASVRHIRPPL
Download Length: 429 bp
>T87 NC_000962:841228-841656 [Mycobacterium tuberculosis H37Rv]
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTCGCGGCTGACGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATC
GACGCATCTTCGAGATTCCGTCACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTT
CCGACGAACCCCGGTCCCAGACACCTCATGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGCGGCCATAGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCT
CGGTGCGCCACATTCGCCCGCCGCTCTAG
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTCGCGGCTGACGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATC
GACGCATCTTCGAGATTCCGTCACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTT
CCGACGAACCCCGGTCCCAGACACCTCATGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGCGGCCATAGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCT
CGGTGCGCCACATTCGCCCGCCGCTCTAG
Antitoxin
Download Length: 86 a.a. Molecular weight: 9114.28 Da Isoelectric Point: 10.6530
>AT87 NP_215262.1 NC_000962:840947-841204 [Mycobacterium tuberculosis H37Rv]
MRTTVSISDEILAAAKRRARERGQSLGAVIEDALRREFAAAHVGGARPTVPVFDGGTGPRRGIDLTSNRALSEVLDEGLE
LNSRK
MRTTVSISDEILAAAKRRARERGQSLGAVIEDALRREFAAAHVGGARPTVPVFDGGTGPRRGIDLTSNRALSEVLDEGLE
LNSRK
Download Length: 258 bp
>AT87 NC_000962:840947-841204 [Mycobacterium tuberculosis H37Rv]
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAGGACGCCCTTCGGCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGAGCGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAGGACGCCCTTCGGCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGAGCGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T125 | Mycobacterium tuberculosis H37Rv |
40.714 |
98.592 |
0.401 |
| T111 | Mycobacterium tuberculosis H37Rv |
36.17 |
99.296 |
0.359 |
| T92 | Mycobacterium tuberculosis H37Rv |
34.058 |
97.183 |
0.331 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT125 | Mycobacterium tuberculosis H37Rv |
35.294 |
100 |
0.353 |
| AT114 | Mycobacterium tuberculosis H37Rv |
42.105 |
77.027 |
0.324 |
| AT76 | Mycobacterium tuberculosis H37Rv |
48.936 |
66.197 |
0.324 |
Multiple sequence alignment
References
(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113]