Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/RHH-PIN |
| Location | 2854267..2854907 | Replicon | chromosome |
| Accession | NC_000962 | ||
| Organism | Mycobacterium tuberculosis H37Rv | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | P9WF63 |
| Locus tag | Rv2530c | Protein ID | NP_217046.1 |
| Coordinates | 2854267..2854686 (-) | Length | 140 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | P9WJ23 |
| Locus tag | Rv2530A | Protein ID | YP_177672.1 |
| Coordinates | 2854683..2854907 (-) | Length | 75 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Rv2525c | 2849852..2850574 | - | 723 | NP_217041.1 | hypothetical protein | - |
| Rv2526 | 2851091..2851318 | + | 228 | NP_217042.1 | antitoxin VapB17 | - |
| Rv2527 | 2851315..2851716 | + | 402 | NP_217043.1 | ribonuclease VapC17 | - |
| Rv2528c | 2851751..2852671 | - | 921 | NP_217044.1 | restriction system protein | - |
| Rv2529 | 2852875..2854266 | + | 1392 | NP_217045.1 | hypothetical protein | - |
| Rv2530c | 2854267..2854686 | - | 420 | NP_217046.1 | ribonuclease VapC39 | Toxin |
| Rv2530A | 2854683..2854907 | - | 225 | YP_177672.1 | antitoxin VapB39 | Antitoxin |
| Rv2531c | 2854938..2857781 | - | 2844 | YP_177889.1 | amino acid decarboxylase | - |
| Rv2532c | 2857853..2858254 | - | 402 | NP_217048.1 | hypothetical protein | - |
| Rv2533c | 2858254..2858724 | - | 471 | NP_217049.1 | N utilization substance protein B | - |
| Rv2534c | 2858727..2859290 | - | 564 | NP_217050.1 | elongation factor P | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 140 a.a. Molecular weight: 14732.70 Da Isoelectric Point: 6.7519
>T114 NP_217046.1 NC_000962:c2854686-2854267 [Mycobacterium tuberculosis H37Rv]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL
Download Length: 420 bp
>T114 NC_000962:c2854686-2854267 [Mycobacterium tuberculosis H37Rv]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG
Antitoxin
Download Length: 75 a.a. Molecular weight: 7993.94 Da Isoelectric Point: 4.0057
>AT114 YP_177672.1 NC_000962:c2854907-2854683 [Mycobacterium tuberculosis H37Rv]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV
Download Length: 225 bp
>AT114 NC_000962:c2854907-2854683 [Mycobacterium tuberculosis H37Rv]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T112 | Mycobacterium tuberculosis H37Rv |
46.259 |
100 |
0.482 |
| T80 | Mycobacterium tuberculosis H37Rv |
40.31 |
96.992 |
0.391 |
| T129 | Mycobacterium tuberculosis H37Rv |
41.353 |
93.662 |
0.387 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT112 | Mycobacterium tuberculosis H37Rv |
52.055 |
100 |
0.521 |
| AT129 | Mycobacterium tuberculosis H37Rv |
37.179 |
100 |
0.392 |
| AT87 | Mycobacterium tuberculosis H37Rv |
42.105 |
77.027 |
0.324 |
Multiple sequence alignment
References
(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113]
(2) Abigail Sharrock et al. (2018) VapC proteins from Mycobacterium tuberculosis share ribonuclease sequence specificity but differ in regulation and toxicity. PloS One 13(8):e0203412. [PubMed:30169502]