Detailed information of TA system TA000080

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 710782..711407
• Replicon: chromosome
• Accession: NC_000962
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF79
• Locus tag: Rv0617
• Protein ID: NP_215131.1
• Coordinates: 711006..711407 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ37
• Locus tag: Rv0616A
• Protein ID: YP_007409270.1
• Coordinates: 710782..711009 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Rv0611c	705961..706182	-	222	NP_215125.2	hypothetical protein	-
Rv0612	706324..706929	+	606	NP_215126.1	hypothetical protein	-
Rv0613c	706948..709515	-	2568	NP_215127.1	hypothetical protein	-
Rv0614	709599..710348	+	750	NP_215128.2	hypothetical protein	-
Rv0615	710345..710587	+	243	NP_215129.1	integral membrane protein	-
Rv0616A	710782..711009	+	228	YP_007409270.1	antitoxin VapB29	Antitoxin
Rv0617	711006..711407	+	402	NP_215131.1	ribonuclease VapC29	Toxin
Rv0620	712716..713807	+	1092	NP_215134.1	galactokinase	-
Rv0621	714202..715266	+	1065	NP_215135.1	membrane protein	-
Rv0622	715370..716317	+	948	NP_215136.2	membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-120)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 13937.76 Da
• Isoelectric Point: 5.7441

>T80 NP_215131.1 NC_000962:711006-711407 [Mycobacterium tuberculosis H37Rv]
VTVLLDANVLIALVVAEHVHHDAAADWLMASDTGFATCPMTQGSLVRFLVRSGQSAAAARDVVSAVQCTSRHEFWPDALS
FAGVEVAGVVGHRQVTDAYLAQLARSHDGQLATLDSGLAHLHGDVAVLIPTTT

Nucleotide

• Length: 402 bp

>T80 NC_000962:711006-711407 [Mycobacterium tuberculosis H37Rv]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGAGCATGTGCATCATGATGCCGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCCAACGACCACCT
GA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 9590.73 Da
• Isoelectric Point: 9.3143

>AT80 YP_007409270.1 NC_000962:710782-711009 [Mycobacterium tuberculosis H37Rv]
VRTTIDLPQDLHKQALAIARDTHRTLSETVADLMRRGLAANRPTALSSDPRTGLPLVSVGTVVTSEDVRSLEDEQ

Nucleotide

• Length: 228 bp

>AT80 NC_000962:710782-711009 [Mycobacterium tuberculosis H37Rv]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGCCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T114	Mycobacterium tuberculosis H37Rv	40.31	96.992	0.391
T112	Mycobacterium tuberculosis H37Rv	39.516	93.233	0.368
T129	Mycobacterium tuberculosis H37Rv	37.903	93.233	0.353
T119	Mycobacterium tuberculosis H37Rv	29.73	100	0.331
T111	Mycobacterium tuberculosis H37Rv	28.777	100	0.301

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: P9WF79

Antitoxin

• AlphaFold DB: P9WJ37

References

(1) Abigail Sharrock et al. (2018) VapC proteins from Mycobacterium tuberculosis share ribonuclease sequence specificity but differ in regulation and toxicity. PloS One 13(8):e0203412. [PubMed:30169502]


 experimental literature