Detailed information of TA system TA000129

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/RHH-PIN
• Location: 3707642..3708316
• Replicon: chromosome
• Accession: NC_000962
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF53
• Locus tag: Rv3320c
• Protein ID: NP_217837.1
• Coordinates: 3707642..3708070 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ17
• Locus tag: Rv3321c
• Protein ID: NP_217838.1
• Coordinates: 3708074..3708316 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Rv3315c	3703464..3703865	-	402	NP_217832.1	cytidine deaminase	-
Rv3316	3704102..3704440	+	339	NP_217833.1	succinate dehydrogenase cytochrome B-556 subunit	-
Rv3317	3704437..3704871	+	435	NP_217834.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
Rv3318	3705000..3706772	+	1773	NP_217835.1	succinate dehydrogenase flavoprotein subunit	-
Rv3319	3706772..3707563	+	792	NP_217836.1	succinate dehydrogenase iron-sulphur protein subunit	-
Rv3320c	3707642..3708070	-	429	NP_217837.1	ribonuclease VapC44	Toxin
Rv3321c	3708074..3708316	-	243	NP_217838.1	antitoxin VapB44	Antitoxin
Rv3322c	3708438..3709052	-	615	YP_177958.1	methyltransferase	-
Rv3323c	3709049..3709714	-	666	YP_177959.1	MoaD-MoaE fusion protein MoaX	-
Rv3324c	3709715..3710248	-	534	NP_217841.3	cyclic pyranopterin monophosphate synthase accessory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T129 NP_217837.1 NC_000962:c3708070-3707642 [Mycobacterium tuberculosis H37Rv]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T129 NC_000962:c3708070-3707642 [Mycobacterium tuberculosis H37Rv]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT129 NP_217838.1 NC_000962:c3708316-3708074 [Mycobacterium tuberculosis H37Rv]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT129 NC_000962:c3708316-3708074 [Mycobacterium tuberculosis H37Rv]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T114	Mycobacterium tuberculosis H37Rv	41.353	93.662	0.387
T112	Mycobacterium tuberculosis H37Rv	41.6	88.652	0.369
T80	Mycobacterium tuberculosis H37Rv	37.903	93.233	0.353

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT114	Mycobacterium tuberculosis H37Rv	37.179	100	0.392
AT92	Mycobacterium tuberculosis H37Rv	34.524	100	0.363

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: P9WF53

Antitoxin

• AlphaFold DB: P9WJ17

References

(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113]


 experimental literature