Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/RHH-PIN |
| Location | 1384278..1384966 | Replicon | chromosome |
| Accession | NC_000962 | ||
| Organism | Mycobacterium tuberculosis H37Rv | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | P9WF69 |
| Locus tag | Rv1242 | Protein ID | NP_215758.1 |
| Coordinates | 1384535..1384966 (+) | Length | 144 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | O50456 |
| Locus tag | Rv1241 | Protein ID | NP_215757.1 |
| Coordinates | 1384278..1384538 (+) | Length | 87 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Rv1237 | 1379855..1380679 | + | 825 | NP_215753.1 | sugar ABC transporter permease SugB | - |
| Rv1238 | 1380684..1381865 | + | 1182 | NP_215754.1 | sugar ABC transporter ATP-binding protein SugC | - |
| Rv1239c | 1381942..1383042 | - | 1101 | NP_215755.1 | magnesium and cobalt transport transmembrane protein CorA | - |
| Rv1240 | 1383213..1384202 | + | 990 | NP_215756.1 | malate dehydrogenase | - |
| Rv1241 | 1384278..1384538 | + | 261 | NP_215757.1 | antitoxin VapB33 | Antitoxin |
| Rv1242 | 1384535..1384966 | + | 432 | NP_215758.1 | ribonuclease VapC33 | Toxin |
| Rv1243c | 1384989..1386677 | - | 1689 | YP_177798.1 | PE-PGRS family protein PE_PGRS23 | - |
| Rv1244 | 1386857..1387717 | + | 861 | NP_215760.1 | lipoprotein LpqZ | - |
| Rv1245c | 1387798..1388628 | - | 831 | NP_215761.1 | short-chain type dehydrogenase/reductase | - |
| Rv1246c | 1388685..1388978 | - | 294 | NP_215762.1 | toxin RelE | - |
| Rv1247c | 1388975..1389244 | - | 270 | NP_215763.1 | antitoxin RelB | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 144 a.a. Molecular weight: 15873.08 Da Isoelectric Point: 5.8838
>T92 NP_215758.1 NC_000962:1384535-1384966 [Mycobacterium tuberculosis H37Rv]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE
Download Length: 432 bp
>T92 NC_000962:1384535-1384966 [Mycobacterium tuberculosis H37Rv]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA
Antitoxin
Download Length: 87 a.a. Molecular weight: 9918.25 Da Isoelectric Point: 6.4755
>AT92 NP_215757.1 NC_000962:1384278-1384538 [Mycobacterium tuberculosis H37Rv]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR
Download Length: 261 bp
>AT92 NC_000962:1384278-1384538 [Mycobacterium tuberculosis H37Rv]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T111 | Mycobacterium tuberculosis H37Rv |
37.681 |
96.503 |
0.364 |
| T87 | Mycobacterium tuberculosis H37Rv |
34.058 |
97.183 |
0.331 |
| T125 | Mycobacterium tuberculosis H37Rv |
31.206 |
98.601 |
0.308 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT111 | Mycobacterium tuberculosis H37Rv |
34.066 |
100 |
0.369 |
| AT129 | Mycobacterium tuberculosis H37Rv |
34.524 |
100 |
0.363 |
| AT125 | Mycobacterium tuberculosis H37Rv |
35.294 |
100 |
0.353 |
Multiple sequence alignment
References
(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113]