Detailed information of TA system TA000111

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/RHH-PIN
• Location: 2364086..2364781
• Replicon: chromosome
• Accession: NC_000962
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: O53501
• Locus tag: Rv2103c
• Protein ID: NP_216619.1
• Coordinates: 2364086..2364520 (-)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ27
• Locus tag: Rv2104c
• Protein ID: NP_216620.1
• Coordinates: 2364527..2364781 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Rv2101	2360240..2363281	+	3042	NP_216617.2	helicase HelZ	-
Rv2102	2363391..2364107	+	717	NP_216618.1	hypothetical protein	-
Rv2103c	2364086..2364520	-	435	NP_216619.1	ribonuclease VapC37	Toxin
Rv2104c	2364527..2364781	-	255	NP_216620.1	antitoxin VapB37	Antitoxin
Rv2104a	2364797..2365054	-	258	YP_009030035.1	hypothetical protein	-
Rv2107	2367359..2367655	+	297	YP_177858.1	PE family protein PE22	-
Rv2108	2367711..2368442	+	732	YP_177859.1	PPE family protein PPE36	-
Rv2109c	2368983..2369729	-	747	NP_216625.1	proteasome subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15662.08 Da
• Isoelectric Point: 7.4681

>T111 NP_216619.1 NC_000962:c2364520-2364086 [Mycobacterium tuberculosis H37Rv]
MKIVDANVLLYAVNTTSEHHKPSLRWLDGALSGADRVGFAWVPLLAFVRLATKVGLFPRPLPREAAITQVADWLAAPSAV
LVNPTVRHADILARMLTYVGTGANLVNDAHLAALAVEHRASIVSYDSDFGRFEGVRWDQPPALL

Nucleotide

• Length: 435 bp

>T111 NC_000962:c2364520-2364086 [Mycobacterium tuberculosis H37Rv]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCACAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9417.57 Da
• Isoelectric Point: 8.9912

>AT111 NP_216620.1 NC_000962:c2364781-2364527 [Mycobacterium tuberculosis H37Rv]
MRTTVTLDDDVEQLVRRRMAERQVSFKKALNDAIRDGASGRPAPSHFSTRTADLGVPAVNLDRALQLAADLEDEELVRRQ
RRGS

Nucleotide

• Length: 255 bp

>AT111 NC_000962:c2364781-2364527 [Mycobacterium tuberculosis H37Rv]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T92	Mycobacterium tuberculosis H37Rv	37.681	96.503	0.364
T87	Mycobacterium tuberculosis H37Rv	36.17	99.296	0.359
T125	Mycobacterium tuberculosis H37Rv	32.857	97.222	0.319
T80	Mycobacterium tuberculosis H37Rv	28.777	100	0.301

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT92	Mycobacterium tuberculosis H37Rv	34.066	100	0.369

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: O53501

Antitoxin

• AlphaFold DB: P9WJ27

References

(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113]


 experimental literature