Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/RHH-PIN |
| Location | 2930070..2930784 | Replicon | chromosome |
| Accession | NC_000962 | ||
| Organism | Mycobacterium tuberculosis H37Rv | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | P9WF59 |
| Locus tag | Rv2602 | Protein ID | NP_217118.1 |
| Coordinates | 2930344..2930784 (+) | Length | 147 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | P9WJ21 |
| Locus tag | Rv2601A | Protein ID | YP_177673.1 |
| Coordinates | 2930070..2930357 (+) | Length | 96 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Rv2595 | 2925492..2925737 | + | 246 | NP_217111.1 | antitoxin VapB40 | - |
| Rv2596 | 2925734..2926138 | + | 405 | NP_217112.1 | ribonuclease VapC40 | - |
| Rv2597 | 2926355..2926975 | + | 621 | NP_217113.1 | membrane protein | - |
| Rv2598 | 2926986..2927480 | + | 495 | NP_217114.1 | hypothetical protein | - |
| Rv2599 | 2927477..2927908 | + | 432 | NP_217115.1 | membrane protein | - |
| Rv2600 | 2927990..2928391 | + | 402 | NP_217116.1 | integral membrane protein | - |
| Rv2601 | 2928388..2929959 | + | 1572 | YP_177892.1 | spermidine synthase | - |
| Rv2601A | 2930070..2930357 | + | 288 | YP_177673.1 | antitoxin VapB41 | Antitoxin |
| Rv2602 | 2930344..2930784 | + | 441 | NP_217118.1 | ribonuclease VapC41 | Toxin |
| Rv2603c | 2930805..2931560 | - | 756 | NP_217119.1 | transcriptional regulator | - |
| Rv2604c | 2931693..2932289 | - | 597 | NP_217120.1 | glutamine amidotransferase SnoP | - |
| Rv2605c | 2932297..2933142 | - | 846 | NP_217121.1 | acyl-CoA thioesterase II | - |
| Rv2606c | 2933171..2934070 | - | 900 | NP_217122.1 | pyridoxine biosynthesis protein | - |
| Rv2607 | 2934198..2934872 | + | 675 | NP_217123.1 | pyridoxine/pyridoxamine 5'-phosphate oxidase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 147 a.a. Molecular weight: 16026.38 Da Isoelectric Point: 6.8599
>T119 NP_217118.1 NC_000962:2930344-2930784 [Mycobacterium tuberculosis H37Rv]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK
Download Length: 441 bp
>T119 NC_000962:2930344-2930784 [Mycobacterium tuberculosis H37Rv]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA
Antitoxin
Download Length: 96 a.a. Molecular weight: 10453.98 Da Isoelectric Point: 7.2797
>AT119 YP_177673.1 NC_000962:2930070-2930357 [Mycobacterium tuberculosis H37Rv]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL
Download Length: 288 bp
>AT119 NC_000962:2930070-2930357 [Mycobacterium tuberculosis H37Rv]
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCGGTGGCGCGGCTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCGGTGGCGCGGCTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T80 | Mycobacterium tuberculosis H37Rv |
29.73 |
100 |
0.331 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113]