Detailed information of TA system TA000076

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/RHH-PIN
• Location: 677710..678329
• Replicon: chromosome
• Accession: NC_000962
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: O53779
• Locus tag: Rv0582
• Protein ID: NP_215096.1
• Coordinates: 677922..678329 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O53778
• Locus tag: Rv0581
• Protein ID: NP_215095.1
• Coordinates: 677710..677925 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Rv0579	676238..676996	+	759	NP_215093.1	hypothetical protein	-
Rv0580c	677125..677616	-	492	NP_215094.1	hypothetical protein	-
Rv0581	677710..677925	+	216	NP_215095.1	antitoxin VapB26	Antitoxin
Rv0582	677922..678329	+	408	NP_215096.1	ribonuclease VapC26	Toxin
Rv0583c	678389..679075	-	687	NP_215097.1	lipoprotein LpqN	-
Rv0584	679229..681862	+	2634	NP_215098.1	glycosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Ribbon-helix-helix protein, CopG (4-39)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14471.43 Da
• Isoelectric Point: 4.4493

>T76 NP_215096.1 NC_000962:677922-678329 [Mycobacterium tuberculosis H37Rv]
VIIDTSALLAYFDAAEPDHAAVSECIDSSADALVVSPYVVAELDYLVATRVGVDAELAVLRELAGGAWELANCGAAEIEQ
AARIVTKYQDQRIGIADAANVVLADRYRTRTILTLDRRHFSALRPIGGGRFTVIP

Nucleotide

• Length: 408 bp

>T76 NC_000962:677922-678329 [Mycobacterium tuberculosis H37Rv]
GTGATCATCGACACGAGTGCGCTGCTTGCCTATTTCGACGCCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTGGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCCTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGTCACGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCGTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7572.66 Da
• Isoelectric Point: 10.5454

>AT76 NP_215095.1 NC_000962:677710-677925 [Mycobacterium tuberculosis H37Rv]
VDKTTVYLPDELKAAVKRAARQRGVSEAQVIRESIRAAVGGAKPPPRGGLYAGSEPIARRVDELLAGFGER

Nucleotide

• Length: 216 bp

>AT76 NC_000962:677710-677925 [Mycobacterium tuberculosis H37Rv]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCGCGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGCAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT87	Mycobacterium tuberculosis H37Rv	48.936	66.197	0.324

Multiple sequence alignment

Structures

Toxin

• PDB: 5X3T

Antitoxin

• PDB: 5X3T
• AlphaFold DB: O53778

References

(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113]

(2) Sung-Min Kang et al. (2017) Functional details of the Mycobacterium tuberculosis VapBC26 toxin-antitoxin system based on a structural study: insights into unique binding and antibiotic peptides. Nucleic Acids Research 45(14):8564-8580. [PubMed:28575388]


 experimental literature
 protein structure literature