Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | txpA-ratA/- |
| Location | 3140274..3140739 | Replicon | chromosome |
| Accession | NC_004668 | ||
| Organism | Enterococcus faecalis V583 | ||
Toxin (Protein)
| Gene name | txpA | Uniprot ID | - |
| Locus tag | EF_3263 | Protein ID | AAO82930.1 |
| Coordinates | 3140379..3140462 (-) | Length | 28 a.a. |
Antitoxin (RNA)
| Gene name | ratA | ||
| Locus tag | EF_3263a | ||
| Coordinates | 3140274..3140739 (+) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EF_RS15395 (EF3258) | 3137280..3137702 | + | 423 | WP_002354745.1 | NusG domain II-containing protein | - |
| EF_RS15400 (EF3259) | 3137714..3138271 | + | 558 | WP_002354743.1 | Gx transporter family protein | - |
| EF_RS15405 (EF3260) | 3138309..3139295 | + | 987 | WP_002372605.1 | polyprenyl synthetase family protein | - |
| EF_RS15410 (EF3261) | 3139601..3139846 | + | 246 | WP_002385128.1 | AbrB family transcriptional regulator | - |
| EF_RS15415 (EF3262) | 3139836..3140201 | + | 366 | WP_002370162.1 | type II toxin-antitoxin system PemK/MazF family toxin | - |
| - | 3140274..3140739 | + | 466 | - | - | Antitoxin |
| - | 3140379..3140462 | - | 84 | - | - | Toxin |
| EF_RS15420 (EF3265) | 3140746..3141537 | - | 792 | WP_002387594.1 | dihydropteroate synthase | - |
| EF_RS15425 (EF3266) | 3141530..3142123 | - | 594 | WP_002379022.1 | non-canonical purine NTP pyrophosphatase | - |
| EF_RS15430 (EF3267) | 3142127..3142696 | - | 570 | WP_002383148.1 | GTP cyclohydrolase I FolE | - |
| EF_RS15435 (EF3268) | 3142690..3143175 | - | 486 | WP_002354734.1 | 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase | - |
| EF_RS15440 (EF3269) | 3143176..3143538 | - | 363 | WP_002354733.1 | dihydroneopterin aldolase | - |
| EF_RS15445 (EF3270) | 3143736..3145085 | - | 1350 | WP_002354732.1 | glutathione-disulfide reductase | - |
| EF_RS15450 (EF3271) | 3145172..3145570 | - | 399 | WP_002358946.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 28 a.a. Molecular weight: 2978.63 Da Isoelectric Point: 10.3237
>T6278 AAO82930.1 NC_004668:c3140462-3140379 [Enterococcus faecalis V583]
VTVFEALMLAIAFATLIVKISNKNDKK
VTVFEALMLAIAFATLIVKISNKNDKK
Download Length: 84 bp
>T6278 NC_004668:c3140462-3140379 [Enterococcus faecalis V583]
GTGACTGTCTTTGAAGCATTGATGCTTGCGATTGCTTTCGCAACATTAATTGTCAAAATCAGTAATAAAAACGACAAAAA
ATAA
GTGACTGTCTTTGAAGCATTGATGCTTGCGATTGCTTTCGCAACATTAATTGTCAAAATCAGTAATAAAAACGACAAAAA
ATAA
Antitoxin
Download Length: 466 bp
>AT6278 NC_004668:3140274-3140739 [Enterococcus faecalis V583]
GAAGAGAGCGTACTAGACATACGCTCTCGGGTAGACCGTTTAAGACGGTAGCTTTAATTTATTGGTTAATAAACCCGTCA
ATCTTCGCTAAAAGTTATGACGGGTTTATTTTTTGTCGTTTTTATTACTGATTTTGACAATTAATGTTGCGAAAGCAATC
GCAAGCATCAATGCTTCAAAGACAGTCACTACTATTCCTTTCTAGGGAAAGAGCAAACAAGCAAAAACATTGGCAGTCCC
CCTTTTTTTGAGAAATTGCCACCGTCATAAACTTCCTACTTGTGTAGTATAACACATTTGTACCTTCCTAGAATACGCTT
ACTTTCCTGATTATTCAACAGCCGCTTTCTTTTGTAATAAAAATCGCGCACCGTTTACTGGGATAAAACGATTCAGCCAA
CTAAAATTTTTTAGACTAAACAGACCGACCATCTTTTTTGATGGTCGGTCTGTTTAGTCTATTGCT
GAAGAGAGCGTACTAGACATACGCTCTCGGGTAGACCGTTTAAGACGGTAGCTTTAATTTATTGGTTAATAAACCCGTCA
ATCTTCGCTAAAAGTTATGACGGGTTTATTTTTTGTCGTTTTTATTACTGATTTTGACAATTAATGTTGCGAAAGCAATC
GCAAGCATCAATGCTTCAAAGACAGTCACTACTATTCCTTTCTAGGGAAAGAGCAAACAAGCAAAAACATTGGCAGTCCC
CCTTTTTTTGAGAAATTGCCACCGTCATAAACTTCCTACTTGTGTAGTATAACACATTTGTACCTTCCTAGAATACGCTT
ACTTTCCTGATTATTCAACAGCCGCTTTCTTTTGTAATAAAAATCGCGCACCGTTTACTGGGATAAAACGATTCAGCCAA
CTAAAATTTTTTAGACTAAACAGACCGACCATCTTTTTTGATGGTCGGTCTGTTTAGTCTATTGCT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10206 | Enterococcus faecalis V583 |
41.935 |
100 |
0.481 |
| T10203 | Enterococcus faecalis V583 |
44.444 |
100 |
0.444 |
| T10201 | Bacillus subtilis subsp. subtilis str. 168 |
40.741 |
100 |
0.407 |
| T6321 | Bacillus subtilis subsp. subtilis str. 168 |
50 |
66.667 |
0.333 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Françoise Wessner et al. (2015) Regulatory crosstalk between type I and type II toxin-antitoxin systems in the human pathogen Enterococcus faecalis. RNA Biology 12(10):1099-108. [PubMed:26305399]