Detailed information of TA system TA203481

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): bsrG-as-bsrH/-
• Location: 2273232..2273522
• Replicon: chromosome
• Accession: NZ_CP075344
• Organism: Bacillus subtilis subsp. subtilis strain A1 - Midalam

Toxin (Protein)

• Gene name: bsrG
• Uniprot ID: L8EAY0
• Locus tag: KIL00_RS11705
• Protein ID: WP_009967548.1
• Coordinates: 2273232..2273348 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	as-bsrH
Locus tag	-
Coordinates	2273343..2273522 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIL00_RS11670 (2269160)	2269160..2269330	-	171	WP_009967544.1	bacteriocin sublancin-168	-
KIL00_RS11675 (2269627)	2269627..2269944	-	318	WP_009967545.1	sublancin immunity protein SunI	-
KIL00_RS11680 (2270046)	2270046..2271301	-	1256	Protein_2258	UV damage repair protein UvrX	-
KIL00_RS11685 (2271294)	2271294..2271590	-	297	WP_250629554.1	YolD-like family protein	-
KIL00_RS11690 (2271794)	2271794..2272129	+	336	WP_004399073.1	hypothetical protein	-
KIL00_RS11695 (2272172)	2272172..2272528	-	357	WP_004398595.1	hypothetical protein	-
KIL00_RS11700 (2272534)	2272534..2273001	-	468	WP_003246138.1	YolA family protein	-
KIL00_RS11705 (2273232)	2273232..2273348	+	117	WP_009967548.1	type I toxin-antitoxin system toxin BsrG	Toxin
- (2273343)	2273343..2273522	-	180	NuclAT_1	-	Antitoxin
- (2273343)	2273343..2273522	-	180	NuclAT_1	-	Antitoxin
- (2273343)	2273343..2273522	-	180	NuclAT_1	-	Antitoxin
- (2273343)	2273343..2273522	-	180	NuclAT_1	-	Antitoxin
KIL00_RS11710 (2273627)	2273627..2274160	-	534	WP_019712872.1	GNAT family protein	-
KIL00_RS11715 (2274196)	2274196..2274774	-	579	WP_213783399.1	SMI1/KNR4 family protein	-
KIL00_RS11720 (2274838)	2274838..2275335	-	498	WP_003246188.1	SMI1/KNR4 family protein	-
KIL00_RS11725 (2275344)	2275344..2277059	-	1716	WP_213783400.1	ribonuclease YeeF family protein	-
KIL00_RS11730 (2277159)	2277159..2277716	-	558	WP_213783401.1	SMI1/KNR4 family protein	-
KIL00_RS11735 (2277851)	2277851..2277985	+	135	WP_234047931.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_000964 Bacillus subtilis subsp. subtilis str. 168 Prophage TA006323, TA006321, TA06323b, TA020252 2151626..2286408 0.001	inside	Prophage	-	-	2151255..2286691	135436

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (1-38)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4336.39 Da
• Isoelectric Point: 10.1022

>T203481 WP_009967548.1 NZ_CP075344:2273232-2273348 [Bacillus subtilis subsp. subtilis]
MTVYESLMIMINFGGLILNTVLLIFNIMMIVTSSQKKK

Nucleotide

• Length: 117 bp

>T203481 NZ_CP100099:181297-181713 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Nucleotide

• Length: 180 bp

>AT203481 NZ_CP075344:c2273522-2273343 [Bacillus subtilis subsp. subtilis]
AATGATACAATTATATAATTATTTTTTGCATTTTGTTTAATTAAATGCATAAAATAAAAAGACCAGGGTGTTGGTAGCAC
CCTGGCTCTGTACAAAAGCTGCCCATCAAAGGGCTTGCTCGTTGTCAAATCTGCGTAGGCCTAACCCTTCAGGTGTCCAA
ACTCAAGGGAAGGTCTATTT

Structures

Toxin

• AlphaFold DB: A0A7Z7EVU9

Antitoxin