Detailed information of TA system TA203477

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): yonT-as-bsrG/-
• Location: 2219423..2219640
• Replicon: chromosome
• Accession: NZ_CP075344
• Organism: Bacillus subtilis subsp. subtilis strain A1 - Midalam

Toxin (Protein)

• Gene name: yonT
• Uniprot ID: O31941
• Locus tag: KIL00_RS11410
• Protein ID: WP_009967515.1
• Coordinates: 2219423..2219599 (-)
• Length: 59 a.a.

Antitoxin (RNA)

Gene name	as-bsrG
Locus tag	-
Coordinates	2219540..2219640 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIL00_RS11380 (2214611)	2214611..2214838	-	228	WP_213783396.1	helix-turn-helix transcriptional regulator	-
KIL00_RS11385 (2215099)	2215099..2216415	-	1317	WP_004399369.1	hypothetical protein	-
KIL00_RS11390 (2216772)	2216772..2217278	-	507	WP_004399486.1	hypothetical protein	-
KIL00_RS11395 (2217606)	2217606..2218838	-	1233	WP_004399445.1	hypothetical protein	-
KIL00_RS11400 (2218920)	2218920..2219108	-	189	WP_004399547.1	hypothetical protein	-
KIL00_RS11405 (2219153)	2219153..2219404	-	252	WP_010886546.1	hypothetical protein	-
KIL00_RS11410 (2219423)	2219423..2219599	-	177	WP_009967515.1	hypothetical protein	Toxin
- (2219540)	2219540..2219640	+	101	NuclAT_2	-	Antitoxin
- (2219540)	2219540..2219640	+	101	NuclAT_2	-	Antitoxin
- (2219540)	2219540..2219640	+	101	NuclAT_2	-	Antitoxin
- (2219540)	2219540..2219640	+	101	NuclAT_2	-	Antitoxin
KIL00_RS11415 (2219974)	2219974..2220585	-	612	WP_009967516.1	lipoprotein	-
KIL00_RS11420 (2220700)	2220700..2221026	-	327	WP_009967517.1	helix-turn-helix transcriptional regulator	-
KIL00_RS11425 (2221979)	2221979..2222173	+	195	WP_004399291.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_000964 Bacillus subtilis subsp. subtilis str. 168 Prophage TA006323, TA006321, TA06323b, TA020252 2151626..2286408 0.001	inside	Prophage	-	-	2151255..2286691	135436

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6882.45 Da
• Isoelectric Point: 12.8833

>T203477 WP_009967515.1 NZ_CP075344:c2219599-2219423 [Bacillus subtilis subsp. subtilis]
VLEKMGIVVAFLISLTVLTINSLTIVEKVRNLKNGTSKKKKRIRKRLRPKRQRQRIRR

Nucleotide

• Length: 177 bp

>T203477 NZ_CP100098:c5014039-5013122 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCATCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Nucleotide

• Length: 101 bp

>AT203477 NZ_CP075344:2219540-2219640 [Bacillus subtilis subsp. subtilis]
GATTGTAAGAACCGTTAAAGATATGAGGAAAGCAACTACGATACCCATTTTCTCAAGCACTATGTACACCTCCTTTCCTA
TGACTCTATTATAACATAATT

Structures

Toxin

• AlphaFold DB: O31941

Antitoxin