Detailed information of TA system TA180035

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): bsrG-as-bsrH/-
• Location: 2995953..2996243
• Replicon: chromosome
• Accession: NZ_CP064093
• Organism: Bacillus subtilis strain N1282-4at

Toxin (Protein)

• Gene name: bsrG
• Uniprot ID: L8EAY0
• Locus tag: IRJ24_RS15850
• Protein ID: WP_009967548.1
• Coordinates: 2996127..2996243 (-)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	as-bsrH
Locus tag	-
Coordinates	2995953..2996132 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRJ24_RS15820 (2991490)	2991490..2991624	-	135	WP_234047931.1	transposase	-
IRJ24_RS15825 (2991759)	2991759..2992316	+	558	WP_003246042.1	SMI1/KNR4 family protein	-
IRJ24_RS15830 (2992416)	2992416..2994131	+	1716	WP_004398855.1	ribonuclease YeeF family protein	-
IRJ24_RS15835 (2994140)	2994140..2994637	+	498	WP_003246188.1	SMI1/KNR4 family protein	-
IRJ24_RS15840 (2994701)	2994701..2995279	+	579	WP_004399123.1	SMI1/KNR4 family protein	-
IRJ24_RS15845 (2995315)	2995315..2995848	+	534	WP_004399156.1	GNAT family protein	-
- (2995953)	2995953..2996132	+	180	NuclAT_1	-	Antitoxin
- (2995953)	2995953..2996132	+	180	NuclAT_1	-	Antitoxin
- (2995953)	2995953..2996132	+	180	NuclAT_1	-	Antitoxin
- (2995953)	2995953..2996132	+	180	NuclAT_1	-	Antitoxin
IRJ24_RS15850 (2996127)	2996127..2996243	-	117	WP_009967548.1	type I toxin-antitoxin system toxin BsrG	Toxin
IRJ24_RS15855 (2996474)	2996474..2996941	+	468	WP_003246138.1	YolA family protein	-
IRJ24_RS15860 (2996947)	2996947..2997303	+	357	WP_004398595.1	hypothetical protein	-
IRJ24_RS15865 (2997346)	2997346..2997681	-	336	WP_004399073.1	hypothetical protein	-
IRJ24_RS15870 (2997855)	2997855..2998187	+	333	WP_004398710.1	YolD-like family protein	-
IRJ24_RS15875 (2998180)	2998180..2999430	+	1251	WP_004398504.1	UV-damage repair protein uvrX	-
IRJ24_RS15880 (2999532)	2999532..2999849	+	318	WP_009967545.1	sublancin immunity protein SunI	-
IRJ24_RS15885 (3000146)	3000146..3000316	+	171	WP_009967544.1	bacteriocin sublancin-168	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_000964 Bacillus subtilis subsp. subtilis str. 168 Prophage TA006323, TA006321, TA06323b, TA020252 2151626..2286408 0	inside	Prophage	-	-	2982784..3118211	135427

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (1-38)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4336.39 Da
• Isoelectric Point: 10.1022

>T180035 WP_009967548.1 NZ_CP064093:c2996243-2996127 [Bacillus subtilis]
MTVYESLMIMINFGGLILNTVLLIFNIMMIVTSSQKKK

Nucleotide

• Length: 117 bp

>T180035 NZ_CP085056:884884-885291 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Nucleotide

• Length: 180 bp

>AT180035 NZ_CP064093:2995953-2996132 [Bacillus subtilis]
AATGATACAATTATATAATTATTTTTTGCATTTTGTTTAATTAAATGCATAAAATAAAAAGACCAGGGTGTTGGTAGCAC
CCTGGCTCTGTACAAAAGCTGCCCATCAAAGGGCTTGCTCGTTGTCAAATCTGCGTAGGCCTAACCCTTCAGGTGTCCAA
ACTCAAGGGAAGGTCTATTT

Structures

Toxin

• AlphaFold DB: A0A7Z7EVU9

Antitoxin